KIRREL1 / NEPH1 recombinant protein

Recombinant Mouse KIRREL1 / NEPH1 Protein (His tag)

Cat. Num. AAA2547110

• Gene Names: Kirrel; Neph1; Kirrel1; 6720469N11Rik
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: KIRREL1 / NEPH1; Recombinant Mouse KIRREL1 / NEPH1 Protein (His tag); Kirrel1; Neph1; KIRREL1 / NEPH1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 789

• Application Notes: The secreted recombinant mouse KIRREL1 consists of 489 amino acids and has a calculated molecular mass of 53.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Leu 48
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for KIRREL1 / NEPH1 recombinant protein

Background: NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and in vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.

Description: A DNA sequence encoding the extracellular domain of mouse KIRREL1 (NP_570937.2) (Met 1-Leu 525) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 70608146
• NCBI GeneID: 170643
• NCBI Accession #: NP_570937.2
• NCBI GenBank Nucleotide #: NM_130867.3
• UniProt Accession #: Q80W68; Q8CIJ4; Q8CJ59; Q923L4
• Molecular Weight: 69,967 Da
• NCBI Official Full Name: kin of IRRE-like protein 1
• NCBI Official Synonym Full Names: kin of IRRE like (Drosophila)
• NCBI Official Symbol: Kirrel
• NCBI Official Synonym Symbols: Neph1; Kirrel1; 6720469N11Rik
• NCBI Protein Information: kin of IRRE-like protein 1
• UniProt Protein Name: Kin of IRRE-like protein 1
• UniProt Gene Name: Kirrel
• UniProt Synonym Gene Names: Kirrel1; Neph1

Uniprot Description

KIRREL: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. Belongs to the immunoglobulin superfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3|3 F1

Cellular Component: cell projection membrane; dendritic shaft; intercellular junction; lipid raft; perinuclear region of cytoplasm; plasma membrane

Molecular Function: myosin binding; protein binding

Biological Process: cell-cell adhesion; excretion; negative regulation of protein amino acid phosphorylation; positive regulation of actin filament polymerization