Transferrin / CD176 / Serotransferrin Active Protein | CD176 active protein

Recombinant Mouse Transferrin / CD176 / Serotransferrin Protein (Fc tag)

Cat. Num. AAA2546773

• Gene Names: Trf; HP; Tf; Tfn; hpx; Cd176; AI266983
• Purity: >90 % as determined by SDS-PAGE
• Synonyms: Transferrin / CD176 / Serotransferrin; Recombinant Mouse Transferrin / CD176 / Serotransferrin Protein (Fc tag); TRF; Tf; CD176 active protein

• Host: HEK293 Cells
• Purity/Purification: >90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween-80.
• Sequence: Met1-His697

• Species: Mouse
• Tag: C-Fc
• Bio Activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancercells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect istypically 5-40 ng/mL.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.It is recommended that sterile water(400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage:; Lyophilized proteins are stable for up to 12 months when stored at -20°C to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.; ; Shipping:; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for CD176 active protein

Background: Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. This glycoprotein is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of Transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte / pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. Transferrins are iron binding transport proteins which bind Fe3+ ion in association with the binding of an anion, usually bicarbonate. This transferrin binds only one Fe3+ ion per protein molecule. Transports iron ions from the hemolymph into the eggs during the vitellogenic stage. Transferrins are iron binding transport proteins which can bind two Fe (3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin loaded with iron encounters with a transferring receptor on cell surface, transferring binds to it and, as a consequence, is transported into the cell in a visicle by receptor-mediated endocytosis. The PH is reduced by hydrogen iron pumps. The lower pH causes transferrin to release its iron ions. The receptor is then transported through the endocytic cycle back to the cell surface, ready for another round of iron uptake. Each transferrin molecule has the ability to carry two iron ions in the ferric form.

Description: A DNA sequence encoding the mouse TRF (Q921I1) (Met1-His697) was expressed with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 20330802
• NCBI GeneID: 22041
• NCBI Accession #: NP_598738.1
• NCBI GenBank Nucleotide #: NM_133977.2
• UniProt Accession #: Q921I1; O35421; Q3UBW7; Q58E69; Q61803; Q62357; Q62358; Q62359; Q63915; Q64515; Q8VII5; Q922C0
• Molecular Weight: Mol Mass: 101.9 kDa; AP Mol Mass: 102 kD
• NCBI Official Full Name: serotransferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: Trf
• NCBI Official Synonym Symbols: HP; Tf; Tfn; hpx; Cd176; AI266983
• NCBI Protein Information: serotransferrin
• UniProt Protein Name: Serotransferrin
• UniProt Gene Name: Tf
• UniProt Synonym Gene Names: Trf; Transferrin

Uniprot Description

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9 F1|9 55.03 cM

Cellular Component: apical plasma membrane; basal plasma membrane; basement membrane; cell surface; coated pit; cytoplasmic vesicle; early endosome; endocytic vesicle; endosome; extracellular region; extracellular space; extrinsic to external side of plasma membrane; late endosome; membrane; perinuclear region of cytoplasm; recycling endosome; vesicle

Molecular Function: ferric iron binding; ferrous iron binding; protein binding

Biological Process: actin filament organization; activation of JNK activity; ferric iron transport; iron ion transport; osteoclast differentiation; positive regulation of bone resorption; positive regulation of myelination; positive regulation of phosphorylation; positive regulation of receptor-mediated endocytosis; positive regulation of transcription, DNA-dependent