Typical Testing Data/Standard Curve (for reference only)
Human Gamma-Aminobutyric Acid B Receptor 1 (gABBR1) ELISA Kit | gABBR1 elisa kit
Human Gamma-Aminobutyric Acid B Receptor 1 (gABBR1) ELISA Kit
Gene Names
GABRB3; ECA5; EIEE43
Reactivity
Human
Synonyms
Gamma-Aminobutyric Acid B Receptor 1 (gABBR1); Human Gamma-Aminobutyric Acid B Receptor 1 (gABBR1) ELISA Kit; GABAB(1e); GABABR1; GABA-BR1; GABBR1-3; GPRC3A; HGB1a; Gamma-aminobutyric acid type B receptor subunit 1; gABBR1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of GABARAPL2. No significant cross-reactivity or interference between GABARAPL2 and analogues was observed.
Sequence Length
473
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Rat Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
78-5,000pg/mL
Sensitivity
31pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GABARAPL2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GABARAPL2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for gABBR1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GABARAPL2 in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GABARAPL2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GABARAPL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GABARAPL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GABARAPL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GABARAPL2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GABARAPL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GABARAPL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GABARAPL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for gABBR1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,379 Da
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit beta-3 isoform 1
NCBI Official Synonym Full Names
gamma-aminobutyric acid type A receptor beta3 subunit
NCBI Official Symbol
GABRB3
NCBI Official Synonym Symbols
ECA5; EIEE43
NCBI Protein Information
gamma-aminobutyric acid receptor subunit beta-3
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit beta-3
Protein Family
UniProt Gene Name
GABRB3
NCBI Description
This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. This gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. This gene may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]
Uniprot Description
Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.
Research Articles on gABBR1
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Product Notes
The Human gABBR1 gabrb3 (Catalog #AAA2104903) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104903 ELISA Kit recognizes Human gABBR1. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the gABBR1 gabrb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gamma-Aminobutyric Acid B Receptor 1 (gABBR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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