CDC37 / CDC37A recombinant protein

Recombinant Mouse CDC37 / CDC37A Protein (His & GST tag)

Cat. Num. AAA2546770

• Gene Names: Cdc37; p50; p50Cdc37
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CDC37 / CDC37A; Recombinant Mouse CDC37 / CDC37A Protein (His & GST tag); CDC37; CDC37 / CDC37A recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4
• Sequence Length: 379

• Application Notes: The recombinant mouse CDC37/GST chimera consists of 616 amino acids and has a calculated molecular mass of 72.4 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CDC37 / CDC37A recombinant protein

Background: CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is though to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified to Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.

Description: A DNA sequence encoding the mouse CDC37 (Q61081) (Met1-Ala379) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7949018
• NCBI GeneID: 12539
• NCBI Accession #: NP_058022.1
• NCBI GenBank Nucleotide #: NM_016742.4
• UniProt Accession #: Q61081; Q3TGP0
• Molecular Weight: 44,593 Da
• NCBI Official Full Name: hsp90 co-chaperone Cdc37
• NCBI Official Synonym Full Names: cell division cycle 37
• NCBI Official Symbol: Cdc37
• NCBI Official Synonym Symbols: p50; p50Cdc37
• NCBI Protein Information: hsp90 co-chaperone Cdc37
• UniProt Protein Name: Hsp90 co-chaperone Cdc37
• UniProt Gene Name: Cdc37

Uniprot Description

CDC37: a molecular chaperone that functions in cell signal transduction. Forms complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. Also interacts with androgen receptor. It is thought to play a critical role in directing Hsp90 to its target kinases.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 9|9 A3

Cellular Component: cytoplasm; cytosol; protein complex

Molecular Function: chaperone binding; heat shock protein binding; Hsp90 protein binding; kinase binding; mitogen-activated protein kinase kinase kinase binding; protein C-terminus binding; protein kinase B binding; protein kinase binding; protein kinase regulator activity; protein-tyrosine kinase activity; unfolded protein binding

Biological Process: protein folding; protein stabilization; regulation of protein kinase activity