FcERI / FCER1A recombinant protein

Recombinant Mouse FcERI / FCER1A Protein (Fc tag)

Cat. Num. AAA2546732

• Gene Names: Fcer1a; FcERI; Fce1a; Fcr-5; fcepsilonri
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: FcERI / FCER1A; Recombinant Mouse FcERI / FCER1A Protein (Fc tag); FCER1A; FcERI / FCER1A recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 250

• Application Notes: The recombinant mouse FCER1A /Fc is a disulfide-linked homodimer. The reduced monomer comprises 422 amino acids and has a predicted molecular mass of 48 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Ala 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FcERI / FCER1A recombinant protein

Background: FcERI, also known as FCER1A, is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit (FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.

Description: A DNA sequence encoding the mouse FCER1A (P20489) (Met1-Gln204) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 226823365
• NCBI GeneID: 14125
• NCBI Accession #: NP_034314.2
• NCBI GenBank Nucleotide #: NM_010184.2
• UniProt Accession #: P20489; E9QLR4
• Molecular Weight: 28,657 Da
• NCBI Official Full Name: high affinity immunoglobulin epsilon receptor subunit alpha
• NCBI Official Synonym Full Names: Fc receptor, IgE, high affinity I, alpha polypeptide
• NCBI Official Symbol: Fcer1a
• NCBI Official Synonym Symbols: FcERI; Fce1a; Fcr-5; fcepsilonri
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit alpha
• UniProt Protein Name: High affinity immunoglobulin epsilon receptor subunit alpha
• UniProt Gene Name: Fcer1a
• UniProt Synonym Gene Names: Fce1a; FcERI

Uniprot Description

FCER1A: Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1 H3|1 80.33 cM

Cellular Component: cell surface; external side of plasma membrane; integral to plasma membrane; lipid raft

Molecular Function: IgE binding; IgE receptor activity; protein binding

Biological Process: activation of JNK activity; activation of MAPK activity; cell surface receptor linked signal transduction; leukotriene biosynthetic process; positive regulation of calcium-mediated signaling; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-3 biosynthetic process; positive regulation of mast cell degranulation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of type I hypersensitivity; serotonin secretion; signal transduction