Rabbit anti-Human Fc epsilon RI Polyclonal Antibody | anti-FCER1A antibody

Anti-Fc epsilon RI Antibody, Rabbit Polyclonal

Cat. Num. AAA8106826

• Gene Names: FCER1A; FCE1A; FcERI
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: Fc epsilon RI; Polyclonal Antibody; Anti-Fc epsilon RI Antibody; Rabbit Polyclonal; FcERI/FCER1A Antibody; Rabbit PAb; Antigen Affinity Purified; Fc fragment of IgE; high affinity I; receptor for; alpha polypeptide; Anti-FCE1A Antibody; Anti-FcERI Antibody; anti-FCER1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human FcERI/FCER1A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FCER1A antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; ICC/IF: 1:1500-1:50,000
• Immunogen: Recombinant Human FcERI/FCER1A Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human FcERI/FCER1A (rh FcERI/FCER1A). FcERI/FCER1A specific IgG was purified by Human FcERI/FCER1A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of FCER1A in A549 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human FCER1A polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane and cytoplasm.)

Western Blot (WB)

(Anti-FCER1A rabbit polyclonal antibody at 1:500 dilution.Lane A: A431 Membrane LysateLane B: 293T Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-FCER1A antibody

FcERI, also known as FCER1A, is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit(FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.

Product Categories/Family for anti-FCER1A antibody

Fc Receptors

References

Shikanai T, et al. (2002) Sequence variants in the FcepsilonRI alpha chain gene. J Appl Physiol. 93(1):37-41.Sada K, et al. (2002) Regulation of FcepsilonRI-mediated degranulation by an adaptor protein 3BP2 in rat basophilic leukemia RBL-2H3 cells. Blood. 100(6):2138-44.Takahashi K, et al. (2003) Transcriptional regulation of the human high affinity IgE receptor alpha-chain gene. Mol Immunol. 38(16-18):1193-9.

NCBI and Uniprot Product Information

• NCBI GI #: 119865
• NCBI GeneID: 2205
• UniProt Accession #: P12319
• Molecular Weight: 30kDa
• NCBI Official Full Name: High affinity immunoglobulin epsilon receptor subunit alpha
• NCBI Official Synonym Full Names: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• NCBI Official Symbol: FCER1A
• NCBI Official Synonym Symbols: FCE1A; FcERI
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E r
• UniProt Protein Name: High affinity immunoglobulin epsilon receptor subunit alpha
• Protein Family: High affinity immunoglobulin epsilon receptor
• UniProt Gene Name: FCER1A
• UniProt Synonym Gene Names: FCE1A; FcERI
• UniProt Entry Name: FCERA_HUMAN

NCBI Description

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

Uniprot Description

Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.