NBL1 / DAND1 / DAN recombinant protein

Recombinant Human NBL1 / DAND1 / DAN Protein (Fc tag)

Cat. Num. AAA2546461

• Gene Names: NBL1; NB; DAN; NO3; DAND1; D1S1733E
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: NBL1 / DAND1 / DAN; Recombinant Human NBL1 / DAND1 / DAN Protein (Fc tag); RP5-1056L3.4; D1S1733E; DAN; DAND1; MGC8972; NB; NO3; NBL-1; NBL1 / DAND1 / DAN recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 180

• Application Notes: The recombinant human DAN/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and has a calculated molecular mass of 44.4 kDa. As a result of glycosylation, rh DAN/Fc monomer migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 16
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NBL1 / DAND1 / DAN recombinant protein

Background: The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotype s of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.

Description: A DNA sequence encoding the human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed with C-terminal fused Fc region of human IgG1.

NCBI and Uniprot Product Information

• NCBI GI #: 4885509
• NCBI GeneID: 4681
• NCBI Accession #: NP_005371.1
• NCBI GenBank Nucleotide #: NM_005380.4
• Molecular Weight: 23,169 Da
• NCBI Official Full Name: neuroblastoma suppressor of tumorigenicity 1 isoform 2
• NCBI Official Synonym Full Names: neuroblastoma 1, DAN family BMP antagonist
• NCBI Official Symbol: NBL1
• NCBI Official Synonym Symbols: NB; DAN; NO3; DAND1; D1S1733E
• NCBI Protein Information: neuroblastoma suppressor of tumorigenicity 1
• UniProt Protein Name: Neuroblastoma suppressor of tumorigenicity 1
• UniProt Gene Name: NBL1
• UniProt Synonym Gene Names: DAN; DAND1

NCBI Description

This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]

Uniprot Description

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.