Human glucocorticoid receptor-beta, GR-beta ELISA Kit | GR-beta elisa kit

Human glucocorticoid receptor-beta, GR-beta ELISA Kit

Cat. Num. AAA702548

• Gene Names: NR3C1; GR; GCR; GRL; GCCR
• Reactivity: Human
• Synonyms: glucocorticoid receptor-beta; GR-beta; Human glucocorticoid receptor-beta; GR-beta ELISA Kit; GR-beta elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GRIN1. No significant cross-reactivity or interference between human GRIN1 and analogues was observed.
• Sequence Length: 742

• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 125 pg/ml-8000 pg/ml
• Sensitivity: 31.25 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GR-beta elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRIN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 66528642
• NCBI GeneID: 2908
• NCBI Accession #: NP_001018661.1
• NCBI GenBank Nucleotide #: NM_001020825.1
• UniProt Accession #: P04150; P04151; Q53EP5; Q6N0A4; A0ZXF9; B0LPG8; D3DQF4
• Molecular Weight: 82,904 Da
• NCBI Official Full Name: glucocorticoid receptor isoform beta
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• NCBI Official Symbol: NR3C1
• NCBI Official Synonym Symbols: GR; GCR; GRL; GCCR
• NCBI Protein Information: glucocorticoid receptor; glucocorticoid nuclear receptor variant 1
• UniProt Protein Name: Glucocorticoid receptor
• UniProt Gene Name: NR3C1
• UniProt Synonym Gene Names: GRL; GR
• UniProt Entry Name: GCR_HUMAN

NCBI Description

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Uniprot Description

Function: Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression.2 PublicationsManual assertion based on experiment in:Ref.37