SerpinF2 / SERPINF2 Recombinant Protein | SERPINF2 recombinant protein

Recombinant Human SerpinF2 / SERPINF2 Protein (His tag)

Cat. Num. AAA2546353

• Gene Names: SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: SerpinF2 / SERPINF2; Recombinant Human SerpinF2 / SERPINF2 Protein (His tag); SerpinF2; AAP; API; PLI; alpha-2 AP; ALPHA-2-PI; SERPINF2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5
• Sequence Length: 491

• Application Notes: The recombinant human SerpinF2 consists of 475 amino acids after removal of the signal peptide and predicts a molecular mass of 53.2 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhSerpinF2 is approximately 70 kDa due to glycosylation.
• Predicted N Terminal: Met 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SERPINF2 recombinant protein

Background: SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta also express this protein at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N- and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin (ogen). It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.

Description: A DNA sequence encoding the human SerpinF2 (NP_000925.2) (Met 1-Lys 491) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 115583663
• NCBI GeneID: 5345
• NCBI Accession #: NP_000925.2
• NCBI GenBank Nucleotide #: NM_000934.3
• UniProt Accession #: P08697; Q8N5U7; Q9UCG2; Q9UCG3; B4E1B7
• Molecular Weight: 47,907 Da
• NCBI Official Full Name: alpha-2-antiplasmin isoform a
• NCBI Official Synonym Full Names: serpin family F member 2
• NCBI Official Symbol: SERPINF2
• NCBI Official Synonym Symbols: AAP; API; PLI; A2AP; ALPHA-2-PI
• NCBI Protein Information: alpha-2-antiplasmin
• UniProt Protein Name: Alpha-2-antiplasmin
• Protein Family: Alpha-2-antiplasmin
• UniProt Gene Name: SERPINF2
• UniProt Synonym Gene Names: AAP; PLI; Alpha-2-AP; Alpha-2-PI

NCBI Description

This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cell surface; extracellular region; extracellular space; fibrinogen complex

Molecular Function: endopeptidase inhibitor activity; protease binding; protein binding; protein homodimerization activity; serine-type endopeptidase inhibitor activity

Biological Process: blood vessel morphogenesis; collagen fibril organization; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; positive regulation of cell differentiation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; renin-angiotensin regulation of blood vessel size

Disease: Alpha-2-plasmin Inhibitor Deficiency