SLPI recombinant protein

Recombinant Human SLPI Protein (His tag)

Cat. Num. AAA2546317

• Gene Names: SLPI; ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: SLPI; Recombinant Human SLPI Protein (His tag); WAP4; WFDC4; SLPI recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4
• Sequence Length: 132

• Application Notes: The secreted recombinant human SLPI consists of 117 amino acids and predicts a molecular mass of 13.1 KDa. The apparent molecular mass of the protein is approximately 15 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Ser 26
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SLPI recombinant protein

Background: Secretory leukoprotease inhibitor (SLPI), also called antileukoprotease (ALP), is a 12-kDa, nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells, including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites, and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract, possessing serine protease inhibitor activity, antibacterial activity, and anti-inflammatory/immunomodulatory activity.

Description: A DNA sequence encoding the human SLPI (P03973) (Met1-Ala132) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 4507065
• NCBI GeneID: 6590
• NCBI Accession #: NP_003055.1
• NCBI GenBank Nucleotide #: NM_003064.3
• UniProt Accession #: P03973; P07757; B2R5H8
• Molecular Weight: 14,326 Da
• NCBI Official Full Name: antileukoproteinase
• NCBI Official Synonym Full Names: secretory leukocyte peptidase inhibitor
• NCBI Official Symbol: SLPI
• NCBI Official Synonym Symbols: ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I
• NCBI Protein Information: antileukoproteinase
• UniProt Protein Name: Antileukoproteinase
• Protein Family: Antileukoproteinase
• UniProt Gene Name: SLPI
• UniProt Synonym Gene Names: WAP4; WFDC4; MPI

NCBI Description

This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes. This antimicrobial protein has antibacterial, antifungal and antiviral activity. [provided by RefSeq, Nov 2014]

Uniprot Description

SLPI: Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular matrix; extracellular space

Molecular Function: endopeptidase inhibitor activity; enzyme binding; protein binding; serine-type endopeptidase inhibitor activity

Biological Process: antibacterial humoral response; immune response; innate immune response; negative regulation of protein binding; negative regulation of viral genome replication; response to lipopolysaccharide