CLK3 recombinant protein

Recombinant Human CLK3 Protein (GST tag)

Cat. Num. AAA2546114

• Gene Names: CLK3; PHCLK3; PHCLK3/152
• Purity: > 89 % as determined by SDS-PAGE
• Synonyms: CLK3; Recombinant Human CLK3 Protein (GST tag); FLJ22858; PHCLK3; PHCLK3/152; CLK3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 89 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM GSH, 0.5mM PMSF, 25% glycerol
• Sequence Length: 490

• Application Notes: The recombinant human CLK3/GST chimera consists of 714 amino acids and predicts a molecular mass of 85 kDa. It migrates as an approxiamtely 70 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CLK3 recombinant protein

Background: Dual specificity protein kinase CLK3, also known as CDC-like kinase 3, and CLK3, is a member of CMGC Ser/Thr protein kinase family and Lammer subfamily. Mammalian CLK is the prototype for a family of dual specificity kinases (termed Lammer kinases) that have been conserved in evolution. CLK family members have shown to interact with, and phosphorylate, serine- and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. The three members of the CLK family of kinases (CLK1, CLK2, and CLK3) have been shown to undergo conserved alternative splicing to generate catalytically active and inactive isoforms. The human CLK2 and CLK3 are found within the nucleus and display dual-specificity kinase activity. The truncated isoforms, hCLK2 (T) and hCLK3 (T), colocalize with SR proteins in nuclear speckles. CLK3 may play a role in the development and progression of azoospermia.

Description: A DNA sequence encoding the human CLK3 isoform b (NP_003983.2) (Met 1-Arg 490) was fused with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 153791372
• NCBI GeneID: 1198
• NCBI Accession #: NP_003983.2
• NCBI GenBank Nucleotide #: NM_003992.4
• UniProt Accession #: P49761; Q53Y48; Q9BRS3; Q9BUJ7; D3DW59
• Molecular Weight: 56,207 Da
• NCBI Official Full Name: dual specificity protein kinase CLK3 isoform b
• NCBI Official Synonym Full Names: CDC like kinase 3
• NCBI Official Symbol: CLK3
• NCBI Official Synonym Symbols: PHCLK3; PHCLK3/152
• NCBI Protein Information: dual specificity protein kinase CLK3
• UniProt Protein Name: Dual specificity protein kinase CLK3
• Protein Family: Dual specificity protein kinase
• UniProt Gene Name: CLK3

NCBI Description

This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. [provided by RefSeq, Jul 2008]

Uniprot Description

CLK3: a CMGC kinase of the CLK family. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May play a role in regulating RNA splicing. Three alternatively spliced isoforms have been described. Isoform 2 is short and lacks the kinase domain. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Protein type: CLK family; CMGC group; EC 2.7.12.1; Kinase, protein; Protein kinase, CMGC; Protein kinase, dual-specificity (non-receptor)

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: intermediate filament cytoskeleton; membrane; nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; protein serine/threonine kinase activity

Biological Process: protein amino acid phosphorylation; regulation of RNA splicing