Rabbit Aggrecan Polyclonal Antibody | anti-ACAN antibody

Rabbit Polyclonal to Human Aggrecan

Cat. Num. AAA243789

• Gene Names: ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
• Reactivity: Gorilla, Hamster, Human, Monkey, Pig; Predicted Reactivity: Mouse, Rat, Bovine, Horse (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: Aggrecan; Polyclonal Antibody; Rabbit Polyclonal to Human Aggrecan; Anti-Aggrecan Antibody (N-Terminus) IHC-plus; ACAN; AGCAN; Aggrecan core protein; Acg1; CSPG1; CSPGCP; CSPCP; AGC1; Aggrecan 1; Large aggregating proteoglycan; MSK16; SEDK; MCSPG; Human Aggrecan; anti-ACAN antibody

• Host: Rabbit
• Reactivity: Gorilla, Hamster, Human, Monkey, Pig; Predicted Reactivity: Mouse, Rat, Bovine, Horse (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human ACAN / Aggrecan. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NCAN (56%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2530

• Applicable Applications for anti-ACAN antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (7 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic 18 amino acid peptide from N-terminus of human ACAN / Aggrecan. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Hamster, Pig (100%); Mouse, Rat, Bovine, Panda, Horse (94%); Dog, Elephant (89%); Bat (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: Aggrecan antibody was raised against synthetic 18 amino acid peptide from N-terminus of human ACAN / Aggrecan. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Hamster, Pig (100%); Mouse, Rat, Bovine, Panda, Horse (94%); Dog, Elephant (89%); Bat (83%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-ACAN / Aggrecan antibody IHC of human bone, cartilage. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-ACAN antibody

Aggrecan Core Protein, ESTs have been isolated from lung, uterus, brain, colon, bone, adrenal, stomach, breast, liver/spleen, fetal lung, fetal testis, and fetal B-cell libraries.

NCBI and Uniprot Product Information

• NCBI GI #: 256017257
• NCBI GeneID: 176
• NCBI Accession #: NP_037359.3
• NCBI GenBank Nucleotide #: NM_013227.3
• UniProt Accession #: P16112; Q13650; Q9UCD3; Q9UCP4; Q9UCP5; Q9UDE0
• Molecular Weight: 239,235 Da
• NCBI Official Full Name: aggrecan core protein isoform 2
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: ACAN
• NCBI Official Synonym Symbols: AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
• NCBI Protein Information: aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1
• UniProt Protein Name: Aggrecan core protein
• Protein Family: Aggrecan core protein
• UniProt Gene Name: ACAN
• UniProt Synonym Gene Names: AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1
• UniProt Entry Name: PGCA_HUMAN

NCBI Description

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; Cell adhesion

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region

Molecular Function: protein binding; metal ion binding; extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding

Biological Process: keratan sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process; cell adhesion; proteolysis; skeletal development

Disease: Spondyloepiphyseal Dysplasia, Kimberley Type; Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type