SFTPD / SP-D / SFTP4 recombinant protein

Recombinant Human SFTPD / SP-D / SFTP4 Protein (His tag)

Cat. Num. AAA2545950

• Gene Names: SFTPD; SP-D; PSP-D; SFTP4; COLEC7
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: SFTPD / SP-D / SFTP4; Recombinant Human SFTPD / SP-D / SFTP4 Protein (His tag); COLEC7; PSP-D; SFTP4; SP-D; SFTPD / SP-D / SFTP4 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 375

• Application Notes: The pro form of human SFTPD consists of 366 amino acids and predictes a molecular mass of 37 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of SFTPD is 47 kDa due to glycosylation.
• Predicted N Terminal: Ala 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for SFTPD / SP-D / SFTP4 recombinant protein

Background: Surfactant pulmonary-associated protein D, also known as SFTPD and SP-D, is a member of the collectin family of C-type lectins that is synthesized in many tissues including respiratory epithelial cells in the lung, and contains one C-type lectin domain and one collagen-like domain. The polymorphic variation in the N-terminal domain of the SP-D molecule influences oligomerization, function, and the concentration of the molecule in serum. SFTPD is produced primarily by alveolar type II cells and nonciliated bronchiolar cells in the lung and is constitutively secreted into the alveoli where it influences surfactant homeostasis, effector cell functions, and host defense. It is upregulated in a variety of inflammatory and infectious conditions including Pneumocystis pneumonia and asthma. SFTPD is humoral molecules of the innate immune system, and is considered a functional candidate in chronic periodontitis. Besides it is involved in the development of acute and chronic inflammation of the lung. Several human lung diseases are characterized by decreased levels of bronchoalveolar SFTPD. Thus, recombinant SFTPD has been proposed as a therapeutical option for cystic fibrosis, neonatal lung disease and smoking-induced emphysema. Furthermore, SFTPD serum levels can be used as disease activity markers for interstitial lung diseases.

Description: A DNA sequence encoding the human SFTPD (NP_003010.4) precursor (Met 1-Phe 375) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 61699226
• NCBI GeneID: 6441
• NCBI Accession #: NP_003010.4
• NCBI GenBank Nucleotide #: NM_003019.4
• UniProt Accession #: P35247; Q5T0M3; Q6FH08; Q86YK9; Q8TCD8; Q9UCJ2; Q9UCJ3
• Molecular Weight: 37,728 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: SFTPD
• NCBI Official Synonym Symbols: SP-D; PSP-D; SFTP4; COLEC7
• NCBI Protein Information: pulmonary surfactant-associated protein D
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• UniProt Gene Name: SFTPD
• UniProt Synonym Gene Names: COLEC7; PSPD; SFTP4; PSP-D; SP-D

NCBI Description

The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]

Uniprot Description

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: clathrin-coated endocytic vesicle; endocytic vesicle; endoplasmic reticulum membrane; extracellular region; lamellar body; lysosome

Molecular Function: carbohydrate binding; protein binding

Biological Process: alveolus development; cellular protein metabolic process; defense response to bacterium; innate immune response; macrophage chemotaxis; negative regulation of interleukin-2 biosynthetic process; negative regulation of T cell proliferation; positive regulation of phagocytosis; receptor-mediated endocytosis; regulation of immune response; surfactant homeostasis