STIM1 / GOK recombinant protein

Recombinant Human STIM1 / GOK Protein (His tag)

Cat. Num. AAA2545780

• Gene Names: STIM1; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: STIM1 / GOK; Recombinant Human STIM1 / GOK Protein (His tag); D11S4896E; GOK; STIM1; STIM1 / GOK recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 685

• Application Notes: The recombinant human STIM1 consists of 202 amino acids and predictes a molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh STIM1 is approximately 33-38 kDa due to glycosylation.
• Predicted N Terminal: Leu 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for STIM1 / GOK recombinant protein

Background: Stromal interaction molecule 1, also known as STIM1 and GOK, is a cell membrane, a single-pass type I  membrane protein and a endoplasmic reticulum membrane protein. STIM1 / GOK is ubiquitously expressed in various human primary cells and tumor cell lines. It contains one EF-hand domain and one SAM (sterile alpha motif) domain. STIM1 / GOK plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. It acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, STIM1 / GOK translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+  (CRAC) channel subunit, TMEM142A / ORAI1. Transfection of STIM1 / GOK into cells derived from a rhabdoid tumor and from a rhabdomyosarcoma that do not express detectable levels of STIM1 can induce cell death, suggesting a possible role in the control of rhabdomyosarcomas and rhabdoid tumors. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.

Description: A DNA sequence encoding the human STIM1 (NP_003147.2) extracellular domain (Met 1-Asp 213) was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 21070997
• NCBI GeneID: 6786
• NCBI Accession #: NP_003147.2
• NCBI GenBank Nucleotide #: NM_003156.3
• UniProt Accession #: Q13586; Q8N382; E9PQJ4
• Molecular Weight: 62,133 Da
• NCBI Official Full Name: stromal interaction molecule 1 isoform 2
• NCBI Official Synonym Full Names: stromal interaction molecule 1
• NCBI Official Symbol: STIM1
• NCBI Official Synonym Symbols: GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
• NCBI Protein Information: stromal interaction molecule 1
• UniProt Protein Name: Stromal interaction molecule 1
• UniProt Gene Name: STIM1

NCBI Description

This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

STIM1: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Ubiquitously expressed in various human primary cells and tumor cell lines.

Protein type: Calcium-binding; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to plasma membrane; sarcoplasmic reticulum membrane

Molecular Function: calcium channel regulator activity; calcium ion binding; identical protein binding; microtubule plus-end binding; protease binding; protein binding

Biological Process: activation of store-operated calcium channel activity; cellular calcium ion homeostasis; detection of calcium ion; regulation of calcium ion transport

Disease: Immunodeficiency 10; Myopathy, Tubular Aggregate, 1