STIM1 recombinant protein

STIM1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120821

• Gene Names: STIM1; GOK; D11S4896E; STIM1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: STIM1; STIM1 Protein; Human; Recombinant (His Tag); Human STIM1/GOK Protein (His Tag); D11S4896E Protein; GOK Protein; IMD10 Protein; STRMK Protein; TAM Protein; TAM1 Protein; stromal interaction molecule 1; STIM1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp213

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human STIM1 (NP_003147.2) extracellular domain (Met 1-Asp 213) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for STIM1 recombinant protein

Background: Stromal interaction molecule 1, also known as STIM1 and GOK, is a cell membrane, a single-pass type I membrane protein and a endoplasmic reticulum membrane protein. STIM1/GOK is ubiquitously expressed in various human primary cells and tumor cell lines. It contains one EF-hand domain and one SAM (sterile alpha motif) domain. STIM1/GOK plays a role in mediating Ca²⁺ influx following depletion of intracellular Ca²⁺ stores. It acts as Ca²⁺ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca²⁺ depletion, STIM1/GOK translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca²⁺ release-activated Ca²⁺ (CRAC) channel subunit, TMEM142A/ORAI1. Transfection of STIM1/GOK into cells derived from a rhabdoid tumor and from a rhabdomyosarcoma that do not express detectable levels of STIM1 can induce cell death, suggesting a possible role in the control of rhabdomyosarcomas and rhabdoid tumors. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.

References

Sabbioni S. et al., 1997, Cancer Res. 57: 4493-7. Manji S.S. et al., 2000, Biochim. Biophys. Acta 1481: 147-55. Williams R.T. et al., 2002, Biochim. Biophys. Acta. 1596: 131-7. Spassova M.A. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 4040-5. Parvez S. et al., 2008, FASEB J. 22: 752-61.

NCBI and Uniprot Product Information

• NCBI GI #: 21070997
• NCBI GeneID: 6786
• UniProt Accession #: Q13586; Q8N382
• NCBI Official Full Name: stromal interaction molecule 1 [Homo sapiens]
• NCBI Official Synonym Full Names: stromal interaction molecule 1
• NCBI Official Symbol: STIM1
• NCBI Official Synonym Symbols: GOK; D11S4896E; STIM1
• UniProt Protein Name: Stromal interaction molecule 1
• Protein Family: Stromal interaction molecule
• UniProt Gene Name: STIM1
• UniProt Synonym Gene Names: GOK
• UniProt Entry Name: STIM1_HUMAN

Uniprot Description

STIM1: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Ubiquitously expressed in various human primary cells and tumor cell lines.

Protein type: Membrane protein, integral; Calcium-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: microtubule; endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: identical protein binding; microtubule plus-end binding; protein binding; calcium ion binding

Biological Process: activation of store-operated calcium channel activity; calcium ion transport; regulation of calcium ion transport; detection of calcium ion; blood coagulation

Disease: Myopathy, Tubular Aggregate, 1; Immunodeficiency 10