Rabbit anti-Human STIM1 Polyclonal Antibody | anti-STIM1 antibody

Anti-STIM1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105335

• Gene Names: STIM1; GOK; D11S4896E; STIM1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: STIM1; Polyclonal Antibody; Anti-STIM1 Antibody; Rabbit Polyclonal; STIM1/GOK Antibody; Rabbit PAb; Antigen Affinity Purified; stromal interaction molecule 1; Anti-D11S4896E Antibody; Anti-GOK Antibody; Anti-IMD10 Antibody; Anti-STRMK Antibody; Anti-TAM Antibody; Anti-TAM1 Antibody; anti-STIM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human STIM1/GOK
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-STIM1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:2000-1:10,000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human STIM1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human STIM1 (rh STIM1). STIM1 specific IgG was purified by human STIM1 affinity chromatography .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human STIM1 in human placenta with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human STIM1 in human breast carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(STIM1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-STIM1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-STIM1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 77 kDaObserved band size: 94 kDa)

Western Blot (WB)

(Anti-STIM1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:77 kDaObserved band size:94 kDa)

Related Product Information for anti-STIM1 antibody

Stromal interaction molecule 1, also known as STIM1 and GOK, is a cell membrane, a single-pass type I membrane protein and a endoplasmic reticulum membrane protein. STIM1/GOK is ubiquitously expressed in various human primary cells and tumor cell lines. It contains one EF-hand domain and one SAM (sterile alpha motif) domain. STIM1/GOK plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. It acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, STIM1/GOK translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/ORAI1. Transfection of STIM1/GOK into cells derived from a rhabdoid tumor and from a rhabdomyosarcoma that do not express detectable levels of STIM1 can induce cell death, suggesting a possible role in the control of rhabdomyosarcomas and rhabdoid tumors. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.

References

Sabbioni S. et al., 1997, Cancer Res. 57: 4493-7. Manji S.S. et al., 2000, Biochim. Biophys. Acta 1481: 147-55. Williams R.T. et al., 2002, Biochim. Biophys. Acta. 1596: 131-7. Spassova M.A. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 4040-5. Parvez S. et al., 2008, FASEB J. 22: 752-61.

NCBI and Uniprot Product Information

• NCBI GI #: 21070997
• NCBI GeneID: 6786
• UniProt Accession #: Q13586; Q8N382
• NCBI Official Full Name: stromal interaction molecule 1 [Homo sapiens]
• NCBI Official Synonym Full Names: stromal interaction molecule 1
• NCBI Official Symbol: STIM1
• NCBI Official Synonym Symbols: GOK; D11S4896E; STIM1
• UniProt Protein Name: Stromal interaction molecule 1
• Protein Family: Stromal interaction molecule
• UniProt Gene Name: STIM1
• UniProt Synonym Gene Names: GOK
• UniProt Entry Name: STIM1_HUMAN

Uniprot Description

STIM1: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Ubiquitously expressed in various human primary cells and tumor cell lines.

Protein type: Membrane protein, integral; Calcium-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: microtubule; endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: identical protein binding; microtubule plus-end binding; protein binding; calcium ion binding

Biological Process: activation of store-operated calcium channel activity; calcium ion transport; regulation of calcium ion transport; detection of calcium ion; blood coagulation

Disease: Myopathy, Tubular Aggregate, 1; Immunodeficiency 10