Neuroligin 1 / NLGN1 Recombinant Protein | NLGN1 recombinant protein

Recombinant Human Neuroligin 1 / NLGN1 Protein (His tag)

Cat. Num. AAA2545722

• Gene Names: NLGN1; NL1
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Neuroligin 1 / NLGN1; Recombinant Human Neuroligin 1 / NLGN1 Protein (His tag); KIAA1070; MGC45115; NL1; NLGN1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 823

• Application Notes: The recombinant human NLGN1 consists of 643 amino acids and predictes a molecular mass of 72 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhNLGN1 is approximately 85-95 kDa due to glycosylation.
• Predicted N Terminal: Gln 46
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NLGN1 recombinant protein

Background: Neuroligin 1 (NLGN1) belongs to the type -B carboxylesterase/lipase family, is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors, channels, and signal-transduction molecules to synaptic sites of cell adhesion. Neuroligins consist of five members (NLGN1, NLGN2, NLGN3, NLGN4 and NLGN4Y), which interact with beta-neurexins and this interaction is involved in the formation of functional synapses. The extracellular domain of functional Neuroligin 1 associates as a dimer when analyzed by sedimentation equilibrium. Neuroligin 1 has a unique N-linked glycosylation pattern in the neuroligin family, and glycosylation and its processing modify neuroligin activity. Neuroligin 1 is a potent trigger for the de novo formation of synaptic connections, and it has recently been suggested that it is required for the maturation of functionally competent excitatory synapses. The persistent expression of Neuroligin 1 is required for the maintenance of NMDAR-mediated synaptic transmission, which enables normal development of synaptic plasticity and long-term memory in the amygdala of adult animals.

Description: A DNA sequence encoding the human NLGN1 (NP_055747.1) extracellular domain (Met 1-Ser 677) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7662470
• NCBI GeneID: 22871
• NCBI Accession #: NP_055747.1
• NCBI GenBank Nucleotide #: NM_014932.3
• UniProt Accession #: Q8N2Q7; Q9UPT2
• Molecular Weight: 92,000 Da
• NCBI Official Full Name: neuroligin-1
• NCBI Official Synonym Full Names: neuroligin 1
• NCBI Official Symbol: NLGN1
• NCBI Official Synonym Symbols: NL1
• NCBI Protein Information: neuroligin-1
• UniProt Protein Name: Neuroligin-1
• Protein Family: Neuroligin
• UniProt Gene Name: NLGN1
• UniProt Synonym Gene Names: KIAA1070

NCBI Description

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. [provided by RefSeq, Jul 2008]

Uniprot Description

NLGN1: Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q26.31

Cellular Component: cell surface; dendrite; dendritic spine; extracellular space; filopodium tip; Golgi apparatus; integral to plasma membrane; plasma membrane; postsynaptic density; synapse

Molecular Function: cell adhesion molecule binding; neurexin binding; PDZ domain binding; protein dimerization activity; receptor activity

Biological Process: calcium-dependent cell-cell adhesion; cytoskeletal matrix organization at active zone; establishment of protein localization; heterophilic cell adhesion; nervous system development; neurite development; neuron adhesion; positive regulation of filopodium formation; positive regulation of synaptic transmission, GABAergic; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; protein homooligomerization; protein targeting; regulation of neuron differentiation; synaptic vesicle targeting; synaptogenesis