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Western Blot (WB) (Western BlotPositive WB detected in:Mouse liver tissue,Mouse kidney tissue,Mouse brain tissueAll lanes: AK5 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,22,61 kDaObserved band size: 64 kDa)

Rabbit anti-Human, Mouse AK5 Polyclonal Antibody | anti-AK5 antibody

AK5 Antibody

Gene Names
AK5; AK6
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
AK5; Polyclonal Antibody; AK5 Antibody; Adenylate kinase isoenzyme 5; ATP-AMP transphosphorylase 5; anti-AK5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
536
Applicable Applications for anti-AK5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant Human Adenylate kinase isoenzyme 5 protein (61-215AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-28508 / sc-28509
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse liver tissue,Mouse kidney tissue,Mouse brain tissueAll lanes: AK5 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,22,61 kDaObserved band size: 64 kDa)

Western Blot (WB) (Western BlotPositive WB detected in:Mouse liver tissue,Mouse kidney tissue,Mouse brain tissueAll lanes: AK5 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,22,61 kDaObserved band size: 64 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain tissue using MBS7051672 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS7051672 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of HepG2 cells using MBS7051672 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG2 cells using MBS7051672 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-AK5 antibody
Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,315 Da
NCBI Official Full Name
adenylate kinase isoenzyme 5 isoform 2
NCBI Official Synonym Full Names
adenylate kinase 5
NCBI Official Symbol
AK5
NCBI Official Synonym Symbols
AK6
NCBI Protein Information
adenylate kinase isoenzyme 5
UniProt Protein Name
Adenylate kinase isoenzyme 5
Protein Family
UniProt Gene Name
AK5
UniProt Synonym Gene Names
AK 5
UniProt Entry Name
KAD5_HUMAN

NCBI Description

This gene encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AK5: Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Belongs to the adenylate kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.4.3; EC 2.7.4.6; Kinase, other; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: cytoplasm; cytosol; microtubule organizing center

Molecular Function: nucleoside diphosphate kinase activity; nucleoside kinase activity

Biological Process: ADP biosynthetic process; dADP biosynthetic process; nucleobase, nucleoside and nucleotide interconversion; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process; pyrimidine ribonucleotide biosynthetic process

Research Articles on AK5

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Product Notes

The AK5 ak5 (Catalog #AAA7051672) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AK5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AK5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 500-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the AK5 ak5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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