Neuroligin 1 Recombinant Protein | NLGN1 recombinant protein

Neuroligin 1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122951

• Gene Names: Nlgn1; NL1; Nlg1; BB179718; mKIAA1070; 6330415N05Rik
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Neuroligin 1; Neuroligin 1 Protein; Mouse; Recombinant (His Tag); Mouse Neuroligin 1/NLGN1 Protein (His Tag); 6330415N05Rik Protein; BB179718 Protein; mKIAA1070 Protein; NL1 Protein; Nlg1 Protein; neuroligin 1; NLGN1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser697

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 46
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse NLGN1 isoform 1 (Q99K10-1) extracellular domain (Met 1-Ser 697) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NLGN1 recombinant protein

Background: Neuroligin 1 (NLGN1) belongs to the type-B carboxylesterase/lipase family, is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors, channels, and signal-transduction molecules to synaptic sites of cell adhesion. Neuroligins consists of five members (NLGN1, NLGN2, NLGN3, NLGN4, and NLGN4Y), which interact with beta-neurexins, and this interaction is involved in the formation of functional synapses. The extracellular domain of functional Neuroligin 1 associates as a dimer when analyzed by sedimentation equilibrium. Neuroligin 1 has a unique N-linked glycosylation pattern in the neuroligin family, and glycosylation and its processing modify neuroligin activity. Neuroligin 1 is a potent trigger for the de novo formation of synaptic connections, and it has recently been suggested that it is required for the maturation of functionally competent excitatory synapses. The persistent expression of Neuroligin 1 is required for the maintenance of NMDAR-mediated synaptic transmission, which enables the normal development of synaptic plasticity and long-term memory in the amygdala of adult animals.

References

Song JY, et al. (1999) Neuroligin 1 is a postsynaptic cell-adhesion molecule of excitatory synapses. Proc Natl Acad Sci U S A. 96(3): 1100-5.Comoletti D, et al. (2003) Characterization of the interaction of a recombinant soluble neuroligin-1 with neurexin-1beta. J Biol Chem. 278(50): 50497-505.Ylisaukko-oja T, et al. (2005) Analysis of four neuroligin genes as candidates for autism. Eur J Hum Genet. 13(12): 1285-92.Kim J, et al. (2008) Neuroligin-1 is required for normal expression of LTP and associative fear memory in the amygdala of adult animals. Proc Natl Acad Sci U S A. 105(26): 9087-92.Schapitz IU, et al. (2010) Neuroligin 1 is dynamically exchanged at postsynaptic sites. J Neurosci. 30(38): 12733-44.

NCBI and Uniprot Product Information

• NCBI GI #: 34447217
• NCBI GeneID: 192167
• NCBI Accession #: NP_619607.2
• NCBI GenBank Nucleotide #: NM_138666.3
• UniProt Accession #: Q99K10
• Molecular Weight: 94,149 Da
• NCBI Official Full Name: neuroligin-1 isoform 1
• NCBI Official Synonym Full Names: neuroligin 1
• NCBI Official Symbol: Nlgn1
• NCBI Official Synonym Symbols: NL1; Nlg1; BB179718; mKIAA1070; 6330415N05Rik
• NCBI Protein Information: neuroligin-1
• UniProt Protein Name: Neuroligin-1
• Protein Family: Neuroligin
• UniProt Gene Name: Nlgn1
• UniProt Synonym Gene Names: Kiaa1070
• UniProt Entry Name: NLGN1_MOUSE