STXBP3 / UNC-18C recombinant protein

Recombinant Human STXBP3 / UNC-18C Protein (His & GST tag)

Cat. Num. AAA2545662

• Gene Names: STXBP3; PSP; MUNC18C; UNC-18C; MUNC18-3
• Purity: > 82 % as determined by SDS-PAGE
• Synonyms: STXBP3 / UNC-18C; Recombinant Human STXBP3 / UNC-18C Protein (His & GST tag); MUNC18-3; MUNC18C; PSP; UNC-18C; STXBP3 / UNC-18C recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 82 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol
• Sequence Length: 592

• Application Notes: The recombinant human STXBP3/GST chimera consists of 829 amino acids and has a calculated molecular mass of 95.6 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for STXBP3 / UNC-18C recombinant protein

Background: Syntaxin-binding protein 3, also known as Platelet Sec1 protein, Protein unc-18 homolog 3, Protein unc-18 homolog C, Unc-18C, Unc18-3 and STXBP3, is a cytoplasm protein which belongs to the STXBP/unc-18/SEC1 family. STXBP3 is expressed in cells that exhibit granule exocytosis, such as neutrophils, mast cells, platelets and endothelial cells. STXBP3, together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking / fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. STXBP3 participates in the consolidation and secretion of secondary and tertiary granules. STXBP3 contains one SEC1 domain. Phosphorylation at Ser129 may stimulate granule release. Human STXBP3 shares 90% aa identity with mouse STXBP3. STXBP3 interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion. Interacts with STX4.

Description: A DNA sequence encoding the human STXBP3 (O00186-1) (Met 1-Glu 592) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 118600975
• NCBI GeneID: 6814
• NCBI Accession #: NP_009200.2
• NCBI GenBank Nucleotide #: NM_007269.3
• UniProt Accession #: O00186; Q53FW1; Q86YJ3; Q9UPD7; A8K269; A8K5K7
• Molecular Weight: 67,764 Da
• NCBI Official Full Name: syntaxin-binding protein 3
• NCBI Official Synonym Full Names: syntaxin binding protein 3
• NCBI Official Symbol: STXBP3
• NCBI Official Synonym Symbols: PSP; MUNC18C; UNC-18C; MUNC18-3
• NCBI Protein Information: syntaxin-binding protein 3
• UniProt Protein Name: Syntaxin-binding protein 3
• UniProt Gene Name: STXBP3
• UniProt Synonym Gene Names: PSP; Unc18-3; Unc-18C

Uniprot Description

STXBP3: a vesicle protein that, together with STX4 and VAMP2, regulates the insulin-dependent movement of GLUT4 and the docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. Reduction in its expression using siRNA impairs insulin-stimulated GLUT4 vesicle exocytosis in 3T3-L1 adipocytes. Phosphorylated by the insulin receptor (InsR) at Tyr521 in vitro, and bound and phosphorylated by the activated InsR within adipocytes and skeletal muscle. Tyrosine phosphorylation on residues Y219 and Y521 correlates with its dissociation from STX4. Phosphorylated by PKC in platelets in response to thrombin stimulation, inhibiting its binding to STX4. Interacts directly with DOC2B at the cell membrane, excluding its interaction with STX4, regulating glucose-stimulated insulin secretion. Belongs to the STXBP/unc-18/SEC1 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytosol; plasma membrane; specific granule

Molecular Function: protein binding; syntaxin binding

Biological Process: negative regulation of calcium ion-dependent exocytosis; neutrophil degranulation