Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using Ras-GRF1 (Phospho-Ser916) Antibody. The picture on the right is blocked with the phospho peptide.)
Rabbit anti-Mouse, Rat Ras-GRF1 Polyclonal Antibody | anti-Ras-GRF1 antibody
Ras-GRF1 (Phospho-Ser916) Antibody
Gene Names
Rasgrf1; Gnrp; Grf1; p190; CDC25; P190-A; CDC25Mm; Grfbeta; AI844718; Ras-GRF1; p190RhoGEF
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
Ras-GRF1; Polyclonal Antibody; Ras-GRF1 (Phospho-Ser916) Antibody; CDC25; GNRP; GRF1; Guanine nucleotide releasing protein; P140 Ras-GRF; RASGRF1; Ras-specific nucleotide exchange factor CDC25; anti-Ras-GRF1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Specificity
Ras-GRF1 (Phospho-Ser916) Antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at Ser916.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1,262
Applicable Applications for anti-Ras-GRF1 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50~1:100
ELISA: 1:20000
ELISA: 1:20000
Target Modification
Phospho
Modification Sites
M:S916; R:S898
Immunogen
The antiserum was produced against synthesized peptide derived from mouse Ras-GRF1 around the phosphorylation site of Ser916.
Immunogen Range
882-931
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using Ras-GRF1 (Phospho-Ser916) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using Ras-GRF1 (Phospho-Ser916) Antibody. The picture on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
145 kDa
NCBI Official Full Name
ras-specific guanine nucleotide-releasing factor 1 isoform 1
NCBI Official Synonym Full Names
RAS protein-specific guanine nucleotide-releasing factor 1
NCBI Official Symbol
Rasgrf1
NCBI Official Synonym Symbols
Gnrp; Grf1; p190; CDC25; P190-A; CDC25Mm; Grfbeta; AI844718; Ras-GRF1; p190RhoGEF
NCBI Protein Information
ras-specific guanine nucleotide-releasing factor 1
UniProt Protein Name
Ras-specific guanine nucleotide-releasing factor 1
UniProt Gene Name
Rasgrf1
UniProt Synonym Gene Names
Cdc25; Grf1; Ras-GRF1; GNRP
Uniprot Description
Promotes the exchange of Ras-bound GDP by GTP.
Research Articles on Ras-GRF1
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Product Notes
The Ras-GRF1 rasgrf1 (Catalog #AAA9502628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ras-GRF1 (Phospho-Ser916) Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ras-GRF1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50~1:100 ELISA: 1:20000. Researchers should empirically determine the suitability of the Ras-GRF1 rasgrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ras-GRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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