Mouse Grp75 Monoclonal Antibody | anti-Grp75 antibody

Grp75 Monoclonal Antibody

Cat. Num. AAA3014474

• Gene Names: HSPA9; CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: ProA affinity purified
• Synonyms: Grp75; Monoclonal Antibody; Grp75 Monoclonal Antibody; GRP-75; MOT; PBP74; HSPA9; GRP75; HSPA9B; mt-HSP70; 75 kDa glucose regulated protein; 75 kDa glucose-regulated protein; CSA; Glucose Regulated Protein; Grp 75; Heat shock 70 kDa protein 9; Heat shock 70kD protein 9; heat shock 70kDa protein 9; Heat shock 70kDa protein 9B; Heat shock protein 74 kDa A; Heat shock protein A; Heat shock protein cognate 74; Hsc74; Hsp74; Hsp74a; Hspa9a; MGC4500; mitochondrial; Mortalin 2; Mortalin; Mortalin perinuclear; Mortalin2; MOT 2; MOT2; Mthsp70; p66 mortalin; P66 MOT; Peptide binding protein 74; Peptide-binding protein 74; Stress 70 protein mitochondrial; Stress 70 protein mitochondrial precursor; Stress-70 protein; anti-Grp75 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Grp75 monoclonal antibody detects endogenous levels of Grp75 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 679

• Applicable Applications for anti-Grp75 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200
• Immunogen: Peptide
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution. Positive control: Lane1: NIH-3T3 Lane2: HepG2 Lane3: K562 Lane4: L929)

Immunocytochemistry (ICC)

(ICC staining Grp75 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-Grp75 antibody

The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation.

NCBI and Uniprot Product Information

• NCBI GI #: 21264428
• NCBI GeneID: 3313
• UniProt Accession #: P38646; P38647; P48721
• Molecular Weight: 74kDa
• NCBI Official Full Name: Stress-70 protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 9
• NCBI Official Symbol: HSPA9
• NCBI Official Synonym Symbols: CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m
• NCBI Protein Information: stress-70 protein, mitochondrial
• UniProt Protein Name: Stress-70 protein, mitochondrial
• UniProt Gene Name: HSPA9
• UniProt Synonym Gene Names: GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
• UniProt Entry Name: GRP75_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

Uniprot Description

HSPA9B: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Belongs to the heat shock protein 70 family.

Protein type: Nucleolus; Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: focal adhesion; mitochondrion; cytoplasm; nucleolus

Molecular Function: protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding

Biological Process: cellular protein metabolic process; protein folding; protein export from nucleus; protein targeting to mitochondrion; negative regulation of apoptosis