USP46 recombinant protein

Recombinant Human / Mouse USP46 Protein (N-SUMO)

Cat. Num. AAA2545436

• Gene Names: Usp46; AI451644; 1190009E20Rik; 2410018I08Rik
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: USP46; Recombinant Human / Mouse USP46 Protein (N-SUMO); USP46 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
• Sequence Length: 366

• Application Notes: The secreted recombinant human USP46 consists of 366 amino acids and predicts a molecular mass of 42.4 KDa. The apparent molecular mass of the protein is approximately 43 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for USP46 recombinant protein

Background: USP46 belongs to the peptidase C19 family, USP12/USP46 subfamily. Deubiquitinating enzymes (DUBs) are a large group of proteases which are also commonly referred to as deubiquitinating peptidases, deubiquitinating isopeptidases, deubiquitinases, ubiquitin proteases, ubiquitin hydrolyases, ubiquitin isopeptidases, or Dubs. They regulate ubiquitin-dependent metabolic pathways by cleaving ubiquitin-protein bonds. They also may act as negative and positive regulators of the ubiquitin system. Besides ubiquitin recycling, they are also involved in processing of ubiquitin precursors, in proofreading of protein ubiquitination and in disassembly of inhibitory ubiquitin chains. USP46 is a deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. It may act by mediating the deubiquitination of GAD1/GAD67. USP46 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity and it is not involved in deubiquitination of monoubiquitinated FANCD2.

Description: A DNA sequence encoding the human USP46 [ (Identical to the mouse USP46 (NP_808229.1)] (Met1-Glu366) was fused with the SUMO tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 29243896
• NCBI GeneID: 69727
• NCBI Accession #: NP_808229.1
• NCBI GenBank Nucleotide #: NM_177561.3
• UniProt Accession #: P62069; Q3ULU5; Q80V95; Q9H7U4; Q9H9T8
• Molecular Weight: 42,442 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 46
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 46
• NCBI Official Symbol: Usp46
• NCBI Official Synonym Symbols: AI451644; 1190009E20Rik; 2410018I08Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 46
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 46
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Usp46

Uniprot Description

USP46: Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2. Belongs to the peptidase C19 family. USP12/USP46 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 5|5 C3.3

Molecular Function: ubiquitin-specific protease activity

Biological Process: adult feeding behavior; behavioral fear response; behavioral response to ethanol; protein deubiquitination; regulation of synaptic transmission, GABAergic; righting reflex