Immunofluorescence (IF)
(MBS9605582 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human NKX24 Polyclonal Antibody | anti-NKX24 antibody
NKX24 Antibody
Gene Names
NKX2-4; NKX2D; NKX2.4
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NKX24; Polyclonal Antibody; NKX24 Antibody; Homeobox protein NK 2 homolog D; Homeobox protein NK-2 homolog D; Homeobox protein Nkx-2.4; Homeobox protein NKX2.4; NK2 homeobox 4; NK2 homolog D; NK2 transcription factor homolog D; NK2 transcription factor related locus 4; Nkx2-4; NKX24_HUMAN; NKX2D; anti-NKX24 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
NKX24 antibody detects endogenous levels of total NKX24
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
354
Applicable Applications for anti-NKX24 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Chicken
Similarity
Pig (100%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605582 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9605582 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa using NKX24 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa using NKX24 antibody.)
Related Product Information for anti-NKX24 antibody
Function: Probable transcription factor.
Similarity: Belongs to the NK-2 homeobox family.
Similarity: Belongs to the NK-2 homeobox family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 40 kD
Predicted: 37 kDa
Predicted: 37 kDa
NCBI Official Full Name
homeobox protein Nkx-2.4
NCBI Official Synonym Full Names
NK2 homeobox 4
NCBI Official Symbol
NKX2-4
NCBI Official Synonym Symbols
NKX2D; NKX2.4
NCBI Protein Information
homeobox protein Nkx-2.4
UniProt Protein Name
Homeobox protein Nkx-2.4
UniProt Gene Name
NKX2-4
UniProt Synonym Gene Names
NKX2D
Uniprot Description
Probable transcription factor.
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Product Notes
The NKX24 nkx2-4 (Catalog #AAA9605582) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NKX24 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NKX24 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the NKX24 nkx2-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKX24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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