GPD1 / Glycerolphosphate Dehydrogenase 1 Recombinant Protein | GPD1 recombinant protein

Recombinant Human GPD1 / Glycerolphosphate Dehydrogenase 1 Protein (His tag)

Cat. Num. AAA2545232

• Gene Names: GPD1; GPD-C; HTGTI; GPDH-C
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: GPD1 / Glycerolphosphate Dehydrogenase 1; Recombinant Human GPD1 / Glycerolphosphate Dehydrogenase 1 Protein (His tag); GPD1; GPD1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% glycerol, pH 8.0
• Sequence Length: 326

• Application Notes: The recombinant human GPD1 consists of 364 amino acids and predicts a molecular mass of 39.4 KDa. It migrates as an approximately 33-37 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for GPD1 recombinant protein

Background: GPD1, also known as glycerolphosphate dehydrogenase 1, is a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. GPD1 catalyzes the reversible redox conversion of dihydroxyacetone phosphate (DHAP), thus plays a critical role in carbohydrate and lipid metabolism. It also reduces nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. Meanwhile, GPD1 and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in GPD1 gene are a cause of transient infantile hypertriglyceridemia.

Description: A DNA sequence encoding the human GPD1 (P21695) (Met1-Met349) was expressed with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 380714668
• NCBI GeneID: 2819
• NCBI Accession #: NP_001244128.1
• NCBI GenBank Nucleotide #: NM_001257199.1
• UniProt Accession #: P21695; Q8N1B0; F8W1L5
• Molecular Weight: 35,140 Da
• NCBI Official Full Name: glycerol-3-phosphate dehydrogenase
• NCBI Official Synonym Full Names: glycerol-3-phosphate dehydrogenase 1
• NCBI Official Symbol: GPD1
• NCBI Official Synonym Symbols: GPD-C; HTGTI; GPDH-C
• NCBI Protein Information: glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
• UniProt Protein Name: Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
• Protein Family: Glycerol-3-phosphate dehydrogenase
• UniProt Gene Name: GPD1
• UniProt Synonym Gene Names: GPD-C; GPDH-C

NCBI Description

This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

GPD1: Defects in GPD1 are a cause of hypertriglyceridemia, transient infantile (HTGTI). An autosomal recessive disorder characterized by onset of moderate to severe transient hypertriglyceridemia in infancy that normalizes with age. The hypertriglyceridemia is associated with hepatomegaly, moderately elevated transaminases, persistent fatty liver, and the development of hepatic fibrosis. Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Protein type: EC 1.1.1.8; Lipid Metabolism - glycerophospholipid; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: cytosol

Molecular Function: glycerol-3-phosphate dehydrogenase (NAD+) activity

Biological Process: phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process

Disease: Hypertriglyceridemia, Transient Infantile