Glycerol 3 Phosphate Dehydrogenase/GPD1 Active Protein | GPD1 active protein

Glycerol 3 Phosphate Dehydrogenase/GPD1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121892

• Gene Names: GPD1; GPD-C; HTGTI; GPDH-C
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Glycerol 3 Phosphate Dehydrogenase/GPD1; Glycerol 3 Phosphate Dehydrogenase/GPD1 Protein; Human; Recombinant (His Tag); Human GPD1/Glycerolphosphate Dehydrogenase 1 Protein (His Tag); GPD-C Protein; GPDH-C Protein; HTGTI Protein; glycerol-3-phosphate dehydrogenase 1 (soluble); GPD1 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Met349

• Species: Human
• Activity: Measured by its ability to catalyze dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate. The specific activity is >400 pmols/min/ug.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human GPD1(P21695)(Met1-Met349) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GPD1 active protein

Background: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. GPD1 is a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. It also reduces nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. Meanwhile, GPD1 and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Diseases associated with GPD1 include Hypertriglyceridemia, Transient Infantile, and Myopathy, Distal, 1.

References

Ou X. et al., 2006, J Mol Biol. 357 (3): 858-69. Ou Xianjin. et al., 2011, Journal of Molecular Biology. 357 (3): 858-69. Harding Jr. et al., 1975, Biochem Journal. 146: 223-9.

NCBI and Uniprot Product Information

• NCBI GI #: 380714668
• NCBI GeneID: 2819
• NCBI Accession #: NP_001244128.1
• NCBI GenBank Nucleotide #: NM_001257199.1
• UniProt Accession #: P21695; Q8N1B0; F8W1L5
• Molecular Weight: 35,140 Da
• NCBI Official Full Name: glycerol-3-phosphate dehydrogenase
• NCBI Official Synonym Full Names: glycerol-3-phosphate dehydrogenase 1 (soluble)
• NCBI Official Symbol: GPD1
• NCBI Official Synonym Symbols: GPD-C; HTGTI; GPDH-C
• NCBI Protein Information: glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic; glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic; glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; glycerophosphate dehydrogenase
• UniProt Protein Name: Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
• Protein Family: Glycerol-3-phosphate dehydrogenase
• UniProt Gene Name: GPD1
• UniProt Synonym Gene Names: GPD-C; GPDH-C
• UniProt Entry Name: GPDA_HUMAN

NCBI Description

This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

GPD1: Defects in GPD1 are a cause of hypertriglyceridemia, transient infantile (HTGTI). An autosomal recessive disorder characterized by onset of moderate to severe transient hypertriglyceridemia in infancy that normalizes with age. The hypertriglyceridemia is associated with hepatomegaly, moderately elevated transaminases, persistent fatty liver, and the development of hepatic fibrosis. Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Protein type: Oxidoreductase; EC 1.1.1.8; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: glycerol-3-phosphate dehydrogenase complex; mitochondrion; cytosol

Molecular Function: glycerol-3-phosphate dehydrogenase (NAD+) activity; protein homodimerization activity; glycerol-3-phosphate dehydrogenase activity; NAD binding

Biological Process: NADH oxidation; phospholipid metabolic process; glycerophospholipid biosynthetic process; positive regulation of glycolysis; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; glycerophosphate shuttle; cellular lipid metabolic process; glycerol-3-phosphate catabolic process; gluconeogenesis

Disease: Hypertriglyceridemia, Transient Infantile