Rabbit anti-Human Glycerol 3 Phosphate Dehydrogenase/GPD1 Polyclonal Antibody | anti-GPD1 antibody

Anti-Glycerol 3 Phosphate Dehydrogenase/GPD1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107601

• Gene Names: GPD1; GPD-C; HTGTI; GPDH-C
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Glycerol 3 Phosphate Dehydrogenase/GPD1; Polyclonal Antibody; Anti-Glycerol 3 Phosphate Dehydrogenase/GPD1 Antibody; Rabbit Polyclonal; GPD1/Glycerolphosphate Dehydrogenase 1 Antibody; Rabbit PAb; Antigen Affinity Purified; glycerol-3-phosphate dehydrogenase 1 (soluble); Anti-GPD-C Antibody; Anti-GPDH-C Antibody; Anti-HTGTI Antibody; anti-GPD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Glycerol 3 Phosphate Dehydrogenase/GPD1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GPD1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human GPD1/Glycerolphosphate Dehydrogenase 1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human GPD1/Glycerolphosphate Dehydrogenase 1 (rh GPD1/Glycerolphosphate Dehydrogenase 1). GPD1/Glycerolphosphate Dehydrogenase 1 specific IgG was purified by Human GPD1/Glycerolphosphate Dehydrogenase 1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-GPD1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:38 kDaObserved band size:35 kDa)

Immunoprecipitation (IP)

(GPD1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-GPD1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GPD1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)

Related Product Information for anti-GPD1 antibody

GPD1 (Glycerol-3-Phosphate Dehydrogenase 1) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. GPD1 is a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. It also reduces nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. Meanwhile, GPD1 and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Diseases associated with GPD1 include Hypertriglyceridemia, Transient Infantile, and Myopathy, Distal, 1.

References

Ou X. et al., 2006, J Mol Biol. 357 (3): 858-69. Ou Xianjin. et al., 2011, Journal of Molecular Biology. 357 (3): 858-69. Harding Jr. et al., 1975, Biochem Journal. 146: 223-9.

NCBI and Uniprot Product Information

• NCBI GI #: 380714668
• NCBI GeneID: 2819
• NCBI Accession #: NP_001244128.1
• NCBI GenBank Nucleotide #: NM_001257199.1
• UniProt Accession #: P21695; Q8N1B0; F8W1L5
• Molecular Weight: 35,140 Da
• NCBI Official Full Name: glycerol-3-phosphate dehydrogenase
• NCBI Official Synonym Full Names: glycerol-3-phosphate dehydrogenase 1 (soluble)
• NCBI Official Symbol: GPD1
• NCBI Official Synonym Symbols: GPD-C; HTGTI; GPDH-C
• NCBI Protein Information: glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic; glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic; glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; glycerophosphate dehydrogenase
• UniProt Protein Name: Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
• Protein Family: Glycerol-3-phosphate dehydrogenase
• UniProt Gene Name: GPD1
• UniProt Synonym Gene Names: GPD-C; GPDH-C
• UniProt Entry Name: GPDA_HUMAN

NCBI Description

This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

GPD1: Defects in GPD1 are a cause of hypertriglyceridemia, transient infantile (HTGTI). An autosomal recessive disorder characterized by onset of moderate to severe transient hypertriglyceridemia in infancy that normalizes with age. The hypertriglyceridemia is associated with hepatomegaly, moderately elevated transaminases, persistent fatty liver, and the development of hepatic fibrosis. Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Protein type: Oxidoreductase; EC 1.1.1.8; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: glycerol-3-phosphate dehydrogenase complex; mitochondrion; cytosol

Molecular Function: glycerol-3-phosphate dehydrogenase (NAD+) activity; protein homodimerization activity; glycerol-3-phosphate dehydrogenase activity; NAD binding

Biological Process: NADH oxidation; phospholipid metabolic process; glycerophospholipid biosynthetic process; positive regulation of glycolysis; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; glycerophosphate shuttle; cellular lipid metabolic process; glycerol-3-phosphate catabolic process; gluconeogenesis

Disease: Hypertriglyceridemia, Transient Infantile