Rabbit anti-Human GPNMB  Monoclonal Antibody | anti-GPNMB  antibody

Rabbit anti Human GPNMB  Monoclonal Antibody

Cat. Num. AAA2544227

• Gene Names: GPNMB; NMB; HGFIN
• Reactivity: Human
• Applications: ELISA
• Synonyms: GPNMB ; Monoclonal Antibody; Rabbit anti Human GPNMB Monoclonal Antibody; anti-GPNMB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 206

• Applicable Applications for anti-GPNMB antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human GPNMB/Osteoactivin.
• Immunogen: Recombinant Human GPNMB/Osteoactivin Protein
• Buffer: 0.2 mum filtered solution in PBS
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-GPNMB  antibody

GPNMB belongs to the PMEL/NMB family, also known as Osteoactivin and Hematopoietic growth factor-inducible neurokinin 1 (HGFIN), is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It is suggested to influence osteoblast maturation, cell adhesion and migration. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin as a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.

NCBI and Uniprot Product Information

• NCBI GI #: 15079530
• NCBI GeneID: 10457
• Molecular Weight: 62,643 Da
• NCBI Official Full Name: GPNMB protein
• NCBI Official Synonym Full Names: glycoprotein nmb
• NCBI Official Symbol: GPNMB
• NCBI Official Synonym Symbols: NMB; HGFIN
• NCBI Protein Information: transmembrane glycoprotein NMB
• UniProt Protein Name: Transmembrane glycoprotein NMB
• UniProt Gene Name: GPNMB
• UniProt Synonym Gene Names: HGFIN; NMB
• UniProt Entry Name: GPNMB_HUMAN

NCBI Description

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: heparin binding; integrin binding; protein binding; receptor agonist activity; syndecan binding

Biological Process: cell adhesion; cell-cell signaling; negative regulation of cell proliferation; negative regulation of cytokine production; negative regulation of T cell activation; negative regulation of T cell proliferation; positive regulation of cell migration; positive regulation of protein amino acid autophosphorylation; positive regulation of protein amino acid phosphorylation; regulation of tissue remodeling; signal transduction