GPNMB / Osteoactivin Recombinant Protein | GPNMB recombinant protein

Recombinant Human GPNMB Protein (aa 1-474, His Tag)

Cat. Num. AAA2545819

• Gene Names: GPNMB; NMB; HGFIN
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Synonyms: GPNMB / Osteoactivin; Recombinant Human GPNMB Protein (aa 1-474; His Tag); Transmembrane Glycoprotein NMB; Transmembrane Glycoprotein HGFIN; GPNMB; HGFIN; NMB; Osteoactivin; GPNMB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 98 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Met 1-Pro 474

• Application Notes: The recombinant human GPNMB consists of 464 amino acids and predictes a molecular mass of 52.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhGPNMB is approximately 85-90 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
• Species: Human
• Tag: C-His
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water(200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C . Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; Shipping: This product is provided as lyophilized powder which is shipped with blue ice.

SDS-PAGE

Related Product Information for GPNMB recombinant protein

Background: GPNMB belongs to the PMEL / NMB family, also known as Osteoactivin and Hematopoietic growth factor-inducible neurokinin 1 ( HGFIN ), is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It is suggested to influence osteoblast maturation, cell adhesion and migration. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin as a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.

NCBI and Uniprot Product Information

• NCBI GI #: 52694752
• NCBI GeneID: 10457
• NCBI Accession #: NP_001005340.1
• NCBI GenBank Nucleotide #: NM_001005340.1
• UniProt Accession #: Q14956; Q6UVX1; Q8N1A1; A4D155
• Molecular Weight: 52.1 kDa
• NCBI Official Full Name: transmembrane glycoprotein NMB isoform a
• NCBI Official Synonym Full Names: glycoprotein nmb
• NCBI Official Symbol: GPNMB
• NCBI Official Synonym Symbols: NMB; HGFIN
• NCBI Protein Information: transmembrane glycoprotein NMB
• UniProt Protein Name: Transmembrane glycoprotein NMB
• Protein Family: Transmembrane glycoprotein
• UniProt Gene Name: GPNMB
• UniProt Synonym Gene Names: HGFIN; NMB

NCBI Description

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: heparin binding; integrin binding; protein binding; receptor agonist activity; syndecan binding

Biological Process: cell adhesion; cell-cell signaling; negative regulation of cell proliferation; negative regulation of cytokine production; negative regulation of T cell activation; negative regulation of T cell proliferation; positive regulation of cell migration; positive regulation of protein amino acid autophosphorylation; positive regulation of protein amino acid phosphorylation; regulation of tissue remodeling; signal transduction