Osteoactivin/GPNMB Recombinant Protein | GPNMB recombinant protein

Osteoactivin/GPNMB Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120781

• Gene Names: GPNMB; NMB; HGFIN
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Osteoactivin/GPNMB; Osteoactivin/GPNMB Protein; Human; Recombinant (hFc Tag); Human GPNMB/Osteoactivin Protein (Fc Tag); HGFIN Protein; NMB Protein; Osteoactivin Protein; glycoprotein (transmembrane) nmb; GPNMB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro474

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human GPNMB (Q14956-2)(Met1-Pro474) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GPNMB recombinant protein

Background: GPNMB belongs to the PMEL/NMB family, also known as Osteoactivin and Hematopoietic growth factor-inducible neurokinin 1 (HGFIN), is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It is suggested to influence osteoblast maturation, cell adhesion, and migration. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin is a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.

References

Pahl MV. et al., 2010, Clin J Am Soc Nephrol. 5(1): 56-61. Abdelmagid SM. et al., 2008, Exp Cell Res. 314(13): 2334-51. Haralanova-Ilieva B. et al., 2005, J Hepatol. 242(4): 565-72. Abdelmagid SM. et al., 2007, J Cell Physiol. 210(1): 26-37. Furochi H. et al., 2007, J Med Invest. 54(3-4): 248-54.

NCBI and Uniprot Product Information

• NCBI GI #: 52694752
• NCBI GeneID: 10457
• NCBI Accession #: NP_001005340.1
• NCBI GenBank Nucleotide #: NM_001005340.1
• UniProt Accession #: Q14956; Q6UVX1; Q8N1A1; A4D155
• Molecular Weight: 62,643 Da
• NCBI Official Full Name: transmembrane glycoprotein NMB isoform a
• NCBI Official Synonym Full Names: glycoprotein (transmembrane) nmb
• NCBI Official Symbol: GPNMB
• NCBI Official Synonym Symbols: NMB; HGFIN
• NCBI Protein Information: transmembrane glycoprotein NMB; glycoprotein nmb-like protein; osteoactivin; transmembrane glycoprotein HGFIN
• UniProt Protein Name: Transmembrane glycoprotein NMB
• Protein Family: Transmembrane glycoprotein
• UniProt Gene Name: GPNMB
• UniProt Synonym Gene Names: HGFIN; NMB
• UniProt Entry Name: GPNMB_HUMAN

NCBI Description

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: integral to plasma membrane; integral to membrane; melanosome

Molecular Function: heparin binding; integrin binding

Biological Process: osteoblast differentiation; negative regulation of cell proliferation; cell adhesion; bone mineralization