Typical Testing Data/Standard Curve (for reference only)
(Human GPNMB/Osteoactivin PicoKine ELISA Kit standard curve )
Human GPNMB/Osteoactivin ELISA Kit | GPNMB elisa kit
Human GPNMB/Osteoactivin PicoKine ELISA Kit
Gene Names
GPNMB; NMB; HGFIN
Reactivity
Human
Synonyms
GPNMB/Osteoactivin; Human GPNMB/Osteoactivin PicoKine ELISA Kit; Transmembrane glycoprotein NMB; glycoprotein (transmembrane) nmb; Glycoprotein (transmembrane) nmb; Glycoprotein nmb; Glycoprotein nmb like protein; Gpnmb; GPNMB_HUMAN; HGFIN; NMB; Osteoactivin; Transmembrane glycoprotein; Transmembrane glycoprotein HGFIN; GPNMB elisa kit
Reactivity
Human
Specificity
Natural and recombinant human GPNMB
Sequence Length
572
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human GPNMB/Osteoactivin PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human GPNMB/Osteoactivin PicoKine ELISA Kit standard curve )
Related Product Information for GPNMB elisa kit
Principle of the assay: human GPNMB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GPNMB has been precoated onto 96-well plates. Standards(NSO, A22-P486) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GPNMB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GPNMB amount of sample captured in plate.
Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,643 Da
NCBI Official Full Name
Transmembrane glycoprotein NMB
NCBI Official Synonym Full Names
glycoprotein (transmembrane) nmb
NCBI Official Symbol
GPNMB
NCBI Official Synonym Symbols
NMB; HGFIN
NCBI Protein Information
transmembrane glycoprotein NMB; osteoactivin; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN
UniProt Protein Name
Transmembrane glycoprotein NMB
Protein Family
UniProt Gene Name
GPNMB
UniProt Synonym Gene Names
HGFIN; NMB
UniProt Entry Name
GPNMB_HUMAN
NCBI Description
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 7p15
Cellular Component: integral to plasma membrane; melanosome; integral to membrane
Molecular Function: integrin binding; heparin binding
Biological Process: osteoblast differentiation; negative regulation of cell proliferation; cell adhesion; bone mineralization
Research Articles on GPNMB
Similar Products
Product Notes
The Human GPNMB gpnmb (Catalog #AAA177336) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177336 ELISA Kit recognizes Human GPNMB. It is sometimes possible for the material contained within the vial of "GPNMB/Osteoactivin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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