Rabbit CHST15 Polyclonal Antibody | anti-CHST15 antibody

CHST15 Antibody

Cat. Num. AAA2520139

• Gene Names: CHST15; BRAG; GALNAC4S-6ST
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Antigen affinity purification
• Synonyms: CHST15; Polyclonal Antibody; CHST15 Antibody; BRAG; KIAA0598; MGC34346; GALNAC4S-6ST; RP11-47G11.1; DKFZp781H1369; anti-CHST15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Sequence Length: 561

• Applicable Applications for anti-CHST15 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:200-1:2000
• Immunogen: Fusion protein of CHST15
• Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 74749920
• NCBI GeneID: 51363
• Molecular Weight: 58,109 Da
• NCBI Official Full Name: Carbohydrate sulfotransferase 15
• NCBI Official Synonym Full Names: carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
• NCBI Official Symbol: CHST15
• NCBI Official Synonym Symbols: BRAG; GALNAC4S-6ST
• NCBI Protein Information: carbohydrate sulfotransferase 15; B cell RAG associated protein (GALNAC4S-6ST); B-cell RAG-associated gene protein; hBRAG
• UniProt Protein Name: Carbohydrate sulfotransferase 15
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST15
• UniProt Synonym Gene Names: BRAG; GALNAC4S6ST; KIAA0598; hBRAG; GalNAc4S-6ST
• UniProt Entry Name: CHSTF_HUMAN

NCBI Description

Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

CHST15: Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.8.2.33; Transferase; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity; 3'-phosphoadenosine 5'-phosphosulfate binding

Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; hexose biosynthetic process; pathogenesis