CHST15 recombinant protein

CHST15 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120645

• Gene Names: CHST15; BRAG; GALNAC4S-6ST
• Purity: >97% as determined by SDS-PAGE
• Synonyms: CHST15; CHST15 Protein; Human; Recombinant (His Tag); Human CHST15/GALNAC4S-6ST/BRAG Protein (His Tag); BRAG Protein; GALNAC4S-6ST Protein; carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15; CHST15 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser99-Thr561

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human BRAG (NP_056976.2) extracellular domain (Ser 99-Thr 561) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CHST15 recombinant protein

Background: Carbohydrate sulfotransferase 15, also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, GalNAc4S-6ST, B-cell RAG-associated gene protein, CHST15 and BRAG, is a single-pass type II membrane protein which belongs to thesulfotransferase 1 family. CHST15/BRAG is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. It is not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. CHST15/BRAG is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15/BRAG may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression.

References

Verkoczy L.K., et al.,(1998), hBRAG, a novel B cell lineage cDNA encoding a type II transmembrane glycoprotein potentially involved in the regulation of recombination activating gene 1 (RAG1). Eur. J. Immunol. 28:2839-2853.Yuki M., et al., (2000), Structure, expression and mutational analysis of the hBRAG gene on 10q in the frequently deleted region in human endometrial cancer.Oncol. Rep. 7:1339-1342.Ohtake S., et al.,(2001), Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene.J. Biol. Chem. 276:43894-43900.

NCBI and Uniprot Product Information

• NCBI GI #: 45505173
• NCBI GeneID: 51363
• NCBI Accession #: NP_056976.2
• NCBI GenBank Nucleotide #: NM_015892.4
• UniProt Accession #: Q7LFX5; O60338; O60474; Q86VM4
• Molecular Weight: 58,109 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 15 isoform 1
• NCBI Official Synonym Full Names: carbohydrate sulfotransferase 15
• NCBI Official Symbol: CHST15
• NCBI Official Synonym Symbols: BRAG; GALNAC4S-6ST
• NCBI Protein Information: carbohydrate sulfotransferase 15
• UniProt Protein Name: Carbohydrate sulfotransferase 15
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST15
• UniProt Synonym Gene Names: BRAG; GALNAC4S6ST; KIAA0598; hBRAG; GalNAc4S-6ST
• UniProt Entry Name: CHSTF_HUMAN

NCBI Description

Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]