Rabbit MCL1 Polyclonal Antibody | anti-MCL1 antibody

MCL1 Antibody

Cat. Num. AAA2518452

• Gene Names: MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antigen affinity purification
• Synonyms: MCL1; Polyclonal Antibody; MCL1 Antibody; BCL2L3; EAT; MCL1L; MCL1S; MGC104264; MGC1839; Mcl-1; TM; anti-MCL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 350

• Applicable Applications for anti-MCL1 antibody: Western Blot (WB)
• Immunogen: C term -peptide of human MCL1
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MCL1 antibody.)

Testing Data #2

Related Product Information for anti-MCL1 antibody

Mcl-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway (1). Similar to other Bcl-2 family members, Mcl-1 localizes to the mitochondria (2), interacts with and antagonizes pro-apoptotic Bcl-2 family members (3), and inhibits apoptosis induced by a number of cytotoxic stimuli (4). Mcl-1 differs from its other family members in its regulation at both the transcriptional and post-translational level. First, Mcl-1 has an extended amino-terminal PEST region, which is responsible for its relatively short half-life (1, 2). Second, unlike other family members, Mcl-1 is rapidly transcribed via a PI3K/Akt dependent pathway, resulting in its increased expression during myeloid differentiation and cytokine stimulation (1, 5-7). Mcl-1 is phosphorylated in response to treatment with phorbol ester, microtubule-damaging agents, oxidative stress, and cytokine withdrawal (8-11). Phosphorylation at Thr163, the conserved MAP kinase/ERK site located within the PEST region, slows Mcl-1 protein turnover (10) but may prime the GSK-3 mediated phosphorylation at Ser159 that leads to Mcl-1 destabilization (11). Mcl-1 deficiency in mice results in peri-implantation lethality (12). In addition, conditional disruption of the corresponding mcl-1 gene shows that Mcl-1 plays an important role in early lymphoid development and in the maintenance of mature lymphocytes (13).

NCBI and Uniprot Product Information

• NCBI GI #: 4235637
• NCBI GeneID: 4170
• Molecular Weight: 28,662 Da
• NCBI Official Full Name: myeloid cell leukemia sequence 1
• NCBI Official Synonym Full Names: myeloid cell leukemia 1
• NCBI Official Symbol: MCL1
• NCBI Official Synonym Symbols: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related)
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• Protein Family: Minichromosome loss protein
• UniProt Gene Name: MCL1
• UniProt Synonym Gene Names: BCL2L3; Bcl2-L-3
• UniProt Entry Name: MCL1_HUMAN

NCBI Description

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Uniprot Description

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Inhibitor; Membrane protein, integral; Channel, misc.; Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; multicellular organismal development; response to cytokine stimulus; cellular homeostasis; cell fate determination