Rat NES ELISA Kit | NES elisa kit

Rat NES(Nesfatin)ELISA Kit

Cat. Num. AAA2516075

• Gene Names: NES; Nbla00170
• Reactivity: Rat
• Synonyms: NES; Rat NES(Nesfatin)ELISA Kit; NES elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinantRatNES. No significant cross-reactivity or interference between RatNES and analogues was observed.
• Sequence Length: 593

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NES elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatNESconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NES. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NESand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NES.You can calculate the concentration of NESin the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148745624
• NCBI GeneID: 10763
• Molecular Weight: 177,439 Da
• NCBI Official Full Name: NES protein, partial
• NCBI Official Synonym Full Names: nestin
• NCBI Official Symbol: NES
• NCBI Official Synonym Symbols: Nbla00170
• NCBI Protein Information: nestin
• UniProt Protein Name: Nestin
• Protein Family: Nestin
• UniProt Gene Name: NES
• UniProt Entry Name: NEST_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

Uniprot Description

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; cell projection morphogenesis; negative regulation of neuron apoptosis; brain development; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development