Rabbit anti-Human, Mouse Bok Polyclonal Antibody | anti-BOK antibody

Bok, BH3 Domain (BOK, BCL2L9, Bcl-2-related ovarian killer protein, Bcl-2-like protein 9) (FITC)

Cat. Num. AAA6278717

• Gene Names: BOK; BOKL; BCL2L9
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Bok; Polyclonal Antibody; BH3 Domain (BOK; BCL2L9; Bcl-2-related ovarian killer protein; Bcl-2-like protein 9) (FITC); BOK; Bcl-2-like protein 9; anti-BOK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-BOK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Peptide selected from the N-terminal region (BH3 domain) of human Bok.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Bok BH3 domain Pab in HL-60 cell lysate. Bok BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-BOK antibody

Bok belongs to the BCL-2 protein family. BCL-2 family members form hetero-or homodimers and act as anti-and pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains all four BCL-2 like domains (BH1, 2, 3 and 4) and is a pro-apoptotic BCL-2 protein identified in the ovary.

Product Categories/Family for anti-BOK antibody

Antibodies; Apoptosis

References

Hsu, S.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 94(23):12401-12406 (1997). Zhang, H., et al., FEBS Lett. 480 (2-3), 311-313 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 14210524
• NCBI GeneID: 666
• NCBI Accession #: NP_115904.1
• NCBI GenBank Nucleotide #: NM_032515.4
• UniProt Accession #: Q9UMX3
• Molecular Weight: 23,280 Da
• NCBI Official Full Name: bcl-2-related ovarian killer protein
• NCBI Official Synonym Full Names: BCL2-related ovarian killer
• NCBI Official Symbol: BOK
• NCBI Official Synonym Symbols: BOKL; BCL2L9
• NCBI Protein Information: bcl-2-related ovarian killer protein; hBOK; bcl2-L-9; bcl-2-like protein 9
• UniProt Protein Name: Bcl-2-related ovarian killer protein
• Protein Family: Bcl-2-related ovarian killer protein
• UniProt Gene Name: BOK
• UniProt Synonym Gene Names: BCL2L9; hBOK; Bcl2-L-9
• UniProt Entry Name: BOK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family. [provided by RefSeq, Sep 2011]

Uniprot Description

BOK: May play a role in apoptosis. Belongs to the Bcl-2 family.

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; mitochondrial membrane; cytoplasm; integral to membrane; nucleus

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: caspase activation; cell proliferation; neuron apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; male gonad development; oligodendrocyte differentiation; brain development