Rat BMP-3 ELISA Kit | BMP-3 elisa kit

Rat BMP-3 (Bone Morphogenetic Protein 3) ELISA Kit

Cat. Num. AAA2515364

• Gene Names: BMP3; BMP-3A
• Reactivity: Rat
• Synonyms: BMP-3; Rat BMP-3 (Bone Morphogenetic Protein 3) ELISA Kit; BMP-3 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes Rat BMP-3 in samples. No significant cross-reactivity or interference between Rat BMP-3 and analogues was observed.
• Sequence Length: 472

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 37.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BMP-3 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BMP-3 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP-3 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat BMP-3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat BMP-3. You can calculate the concentration of Rat BMP-3 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126507087
• NCBI GeneID: 651
• NCBI Accession #: NP_001192.2
• NCBI GenBank Nucleotide #: NM_001201.2
• UniProt Accession #: P12645; Q4VAS5
• Molecular Weight: 53,372 Da
• NCBI Official Full Name: bone morphogenetic protein 3 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 3
• NCBI Official Symbol: BMP3
• NCBI Official Synonym Symbols: BMP-3A
• NCBI Protein Information: bone morphogenetic protein 3; BMP-3; bone morphogenetic protein 3 (osteogenic); bone morphogenetic protein 3A; bone morphogenetic protein-3; osteogenin
• UniProt Protein Name: Bone morphogenetic protein 3
• UniProt Gene Name: BMP3
• UniProt Synonym Gene Names: BMP3A; BMP-3; BMP-3A
• UniProt Entry Name: BMP3_HUMAN

NCBI Description

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein, also known as osteogenin, induces bone formation. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP3: Negatively regulates bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: regulation of apoptosis; ossification; cell-cell signaling; cartilage development; regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; cell development; skeletal development; growth