Chicken FOXP3 ELISA Kit | FOXP3 elisa kit

Chicken FOXP3 (Forkhead Box Protein P3) ELISA Kit

Cat. Num. AAA2511680

• Gene Names: FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
• Reactivity: Chicken
• Synonyms: FOXP3; Chicken FOXP3 (Forkhead Box Protein P3) ELISA Kit; FOXP3 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken FOXP3. No significant cross-reactivity or interference between Chicken FOXP3 and analogues was observed.
• Sequence Length: 431

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken FOXP3 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXP3 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FOXP3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FOXP3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FOXP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FOXP3. You can calculate the concentration of FOXP3 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31982943
• NCBI GeneID: 50943
• NCBI Accession #: NP_054728.2
• NCBI GenBank Nucleotide #: NM_014009.3
• UniProt Accession #: Q9BZS1; O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0; B9UN80
• Molecular Weight: 44,407 Da
• NCBI Official Full Name: forkhead box protein P3 isoform a
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: FOXP3
• NCBI Official Synonym Symbols: JM2; AIID; IPEX; PIDX; XPID; DIETER
• NCBI Protein Information: forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; scurfin
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP3
• UniProt Synonym Gene Names: IPEX
• UniProt Entry Name: FOXP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell cycle regulation; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: protein complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; histone acetyltransferase binding; protein binding; NFAT protein binding; protein homodimerization activity; sequence-specific DNA binding; histone deacetylase binding; metal ion binding; transcription factor activity; transcription corepressor activity

Biological Process: negative regulation of interleukin-10 production; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; myeloid cell homeostasis; T cell receptor signaling pathway; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; T cell homeostasis; tolerance induction to self antigen; response to virus; negative regulation of transcription factor activity; negative regulation of immune response; cytokine production; negative regulation of T cell cytokine production; positive regulation of peripheral T cell tolerance induction; inhibition of NF-kappaB transcription factor; regulation of T cell anergy; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; negative regulation of cytokine biosynthetic process; transcription from RNA polymerase II promoter; negative regulation of interleukin-4 production; T cell activation; negative regulation of activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of histone acetylation; negative regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-4 production; negative regulation of cell proliferation; negative regulation of isotype switching to IgE isotypes; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of T cell proliferation; B cell homeostasis; regulation of transcription, DNA-dependent; negative regulation of interferon-gamma production; regulation of isotype switching to IgG isotypes; positive regulation of T cell anergy; positive regulation of transforming growth factor-beta1 production; negative regulation of chronic inflammatory response; negative regulation of histone deacetylation; T cell mediated immunity; positive regulation of immature T cell proliferation in the thymus; negative regulation of tumor necrosis factor production; chromatin remodeling; negative regulation of interleukin-17 production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; negative regulation of interleukin-2 biosynthetic process; positive regulation of histone acetylation

Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked