Typical Testing Data/Standard Curve (for reference only)
Guinea Pig FOXP3 ELISA Kit | FOXP3 elisa kit
Guinea pig FOXP3 (Forkhead Box Protein P3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Guinea pig FOXP3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Guinea pig FOXP3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Guinea pig FOXP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Guinea pig FOXP3. You can calculate the concentration of Guinea pig FOXP3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; C2H2-type zinc finger protein; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: protein complex; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; histone acetyltransferase binding; protein binding; protein homodimerization activity; NFAT protein binding; sequence-specific DNA binding; histone deacetylase binding; metal ion binding; transcription corepressor activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; negative regulation of interleukin-4 production; T cell activation; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; positive regulation of transcription, DNA-dependent; myeloid cell homeostasis; negative regulation of cytokine secretion; negative regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; negative regulation of histone acetylation; negative regulation of interleukin-2 production; negative regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-4 production; negative regulation of cell proliferation; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; B cell homeostasis; regulation of transcription, DNA-dependent; negative regulation of interferon-gamma production; negative regulation of interleukin-6 production; T cell homeostasis; positive regulation of T cell anergy; regulation of isotype switching to IgG isotypes; tolerance induction to self antigen; positive regulation of transforming growth factor-beta1 production; negative regulation of chronic inflammatory response; negative regulation of histone deacetylation; negative regulation of transcription factor activity; response to virus; T cell mediated immunity; negative regulation of immune response; cytokine production; positive regulation of immature T cell proliferation in the thymus; negative regulation of T cell cytokine production; negative regulation of tumor necrosis factor production; positive regulation of peripheral T cell tolerance induction; chromatin remodeling; negative regulation of interleukin-17 production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; inhibition of NF-kappaB transcription factor; negative regulation of interleukin-2 biosynthetic process; regulation of T cell anergy; negative regulation of interleukin-5 production; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; negative regulation of cytokine biosynthetic process
Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked
Research Articles on FOXP3
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Product Notes
The Guinea Pig FOXP3 foxp3 (Catalog #AAA2513311) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513311 ELISA Kit recognizes Guinea Pig FOXP3. It is sometimes possible for the material contained within the vial of "FOXP3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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