Human Forkhead box protein P3 ELISA Kit | FOXP3 elisa kit

Human Forkhead box protein P3 ELISA Kit

Cat. Num. AAA763499

• Gene Names: FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
• Reactivity: Human
• Synonyms: Forkhead box protein P3; Human Forkhead box protein P3 ELISA Kit; FOXP3 (Forkhead box protein P3)/DIETER/IPEX/JM2/PIDX/SCURFIN/XPID/AIID/forkhead box P3/FOXP3delta7/immune dysregulation; polyendocrinopathy; enteropathy; X-linked/Scurfin/XPIDpolyendocrinopathy; X-linked; FOXP3 elisa kit

• Reactivity: Human
• Sequence Length: 396

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FOXP3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human FOXP3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FOXP3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FOXP3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human FOXP3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 167466190
• NCBI GeneID: 50943
• NCBI Accession #: NP_001107849.1
• NCBI GenBank Nucleotide #: NM_001114377.1
• Molecular Weight: 44,407 Da
• NCBI Official Full Name: forkhead box protein P3 isoform b
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: FOXP3
• NCBI Official Synonym Symbols: JM2; AIID; IPEX; PIDX; XPID; DIETER
• NCBI Protein Information: forkhead box protein P3
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP3
• UniProt Synonym Gene Names: IPEX
• UniProt Entry Name: FOXP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Cell cycle regulation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytoplasm; nucleus; protein complex

Molecular Function: histone acetyltransferase binding; histone deacetylase binding; metal ion binding; NF-kappaB binding; NFAT protein binding; protein binding; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: anatomical structure morphogenesis; B cell homeostasis; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; chromatin remodeling; cytokine production; inhibition of CREB transcription factor; inhibition of NF-kappaB transcription factor; myeloid cell homeostasis; negative regulation of activated T cell proliferation; negative regulation of cell proliferation; negative regulation of chronic inflammatory response; negative regulation of cytokine biosynthetic process; negative regulation of cytokine secretion; negative regulation of histone acetylation; negative regulation of histone deacetylation; negative regulation of immune response; negative regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; negative regulation of interleukin-17 production; negative regulation of interleukin-2 biosynthetic process; negative regulation of interleukin-2 production; negative regulation of interleukin-4 production; negative regulation of interleukin-5 production; negative regulation of interleukin-6 production; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell cytokine production; negative regulation of T cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of tumor necrosis factor production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of histone acetylation; positive regulation of immature T cell proliferation in the thymus; positive regulation of interleukin-4 production; positive regulation of peripheral T cell tolerance induction; positive regulation of T cell anergy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor-beta1 production; regulation of isotype switching to IgG isotypes; regulation of T cell anergy; regulation of transcription, DNA-dependent; response to virus; T cell activation; T cell homeostasis; T cell mediated immunity; T cell receptor signaling pathway; tolerance induction to self antigen; transcription, DNA-dependent

Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked