Mouse CK-MB ELISA Kit | CK-MB elisa kit

Mouse CK-MB (Creatine Kinase MB Isoenzyme) ELISA Kit

Cat. Num. AAA2511621

• Gene Names: CKM; CKMM; M-CK
• Reactivity: Mouse
• Synonyms: CK-MB; Mouse CK-MB (Creatine Kinase MB Isoenzyme) ELISA Kit; CK-MB elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes Mouse CKMB in samples. No significant cross-reactivity or interference between Mouse CKMB and analogues was observed.
• Sequence Length: 381

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.13-5000pg/mL
• Sensitivity: 46.88pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CKMB were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CKMB were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CK-MB elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CKMB concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CKMB. You can calculate the concentration of Mouse CKMB in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21536288
• NCBI GeneID: 1158
• NCBI Accession #: NP_001815.2
• NCBI GenBank Nucleotide #: NM_001824.4
• UniProt Accession #: P06732; Q96QL9
• Molecular Weight: 43,101 Da
• NCBI Official Full Name: creatine kinase M-type
• NCBI Official Synonym Full Names: creatine kinase, muscle
• NCBI Official Symbol: CKM
• NCBI Official Synonym Symbols: CKMM; M-CK
• NCBI Protein Information: creatine kinase M-type; creatine kinase M chain
• UniProt Protein Name: Creatine kinase M-type
• UniProt Gene Name: CKM
• UniProt Synonym Gene Names: CKMM
• UniProt Entry Name: KCRM_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

Uniprot Description

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation