Western Blot (WB)
(Western blot analysis of CWC15 Antibody (Center) in CHO cell line lysates (35ug/lane). CWC15 (arrow) was detected using the purified Pab.)
Rabbit CWC15 Polyclonal Antibody | anti-CWC15 antibody
CWC15 Antibody (Center)
Gene Names
CWC15; ORF5; AD002; Cwf15; C11orf5; HSPC148
Reactivity
Human, hamster (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CWC15; Polyclonal Antibody; CWC15 Antibody (Center); Spliceosome-associated protein CWC15 homolog; C11orf5; anti-CWC15 antibody
Host
Rabbit
Reactivity
Human, hamster (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CWC15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-152 amino acids from the Central region of human CWC15.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
123-152
Sequence Length
229
Applicable Applications for anti-CWC15 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of CWC15 Antibody (Center) in CHO cell line lysates (35ug/lane). CWC15 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CWC15 Antibody (Center) in CHO cell line lysates (35ug/lane). CWC15 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(CWC15 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CWC15 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CWC15 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CWC15 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-CWC15 antibody
CWC15 involved in pre-mRNA splicing.
References
Anney, R.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1369-1378 (2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Ohi, M.D., et al. Mol. Cell. Biol. 22(7):2011-2024(2002)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Ohi, M.D., et al. Mol. Cell. Biol. 22(7):2011-2024(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26624
NCBI Official Full Name
spliceosome-associated protein CWC15 homolog
NCBI Official Synonym Full Names
CWC15 spliceosome-associated protein
NCBI Official Symbol
CWC15
NCBI Official Synonym Symbols
ORF5; AD002; Cwf15; C11orf5; HSPC148
NCBI Protein Information
spliceosome-associated protein CWC15 homolog
UniProt Protein Name
Spliceosome-associated protein CWC15 homolog
Protein Family
UniProt Gene Name
CWC15
UniProt Synonym Gene Names
C11orf5
UniProt Entry Name
CWC15_HUMAN
Uniprot Description
HSPC148: Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Belongs to the CWC15 family.
Protein type: RNA splicing; Spliceosome
Chromosomal Location of Human Ortholog: 11q21
Cellular Component: nucleoplasm; spliceosome; nucleus
Molecular Function: protein binding; RNA binding
Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression
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Product Notes
The CWC15 cwc15 (Catalog #AAA9201062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 123-152. The CWC15 Antibody (Center) reacts with Human, hamster (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's CWC15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the CWC15 cwc15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CWC15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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