Western Blot (WB)
(MIOX Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MIOX antibody detected the MIOX protein (arrow).)
Rabbit anti-Human MIOX Polyclonal Antibody | anti-MIOX antibody
MIOX, NT (MIOX, ALDRL6, KSP32, RSOR, Inositol oxygenase, Aldehyde reductase-like 6, Kidney-specific protein 32, Myo-inositol oxygenase, Renal-specific oxidoreductase) (Biotin)
Gene Names
MIOX; ALDRL6
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MIOX; Polyclonal Antibody; NT (MIOX; ALDRL6; KSP32; RSOR; Inositol oxygenase; Aldehyde reductase-like 6; Kidney-specific protein 32; Myo-inositol oxygenase; Renal-specific oxidoreductase) (Biotin); Renal-specific oxidoreductase; anti-MIOX antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-MIOX antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
MIOX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MIOX.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MIOX Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MIOX antibody detected the MIOX protein (arrow).)
Western Blot (WB)
(MIOX Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MIOX antibody detected the MIOX protein (arrow).)
Immunohistochemistry (IHC)
(MIOX Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIOX Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MIOX Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIOX Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-MIOX antibody
The specific function of this protein remains unknown.
Product Categories/Family for anti-MIOX antibody
References
Yang, B., et al. J. Diabetes Complicat. (2009). In press: Prabhu, K.S., et al. J. Biol. Chem. 280(20):19895-19901(2005). Arner, R.J., et al. Biochem. Biophys. Res. Commun. 324(4):1386-1392(2004). Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004): Arner, R.J., et al. Biochem. J. 360 (PT 2), 313-320 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,516 Da
NCBI Official Full Name
inositol oxygenase
NCBI Official Synonym Full Names
myo-inositol oxygenase
NCBI Official Symbol
MIOX
NCBI Official Synonym Symbols
ALDRL6
NCBI Protein Information
inositol oxygenase
UniProt Protein Name
Inositol oxygenase
Protein Family
UniProt Gene Name
MIOX
UniProt Synonym Gene Names
ALDRL6; KSP32; RSOR; MI oxygenase
UniProt Entry Name
MIOX_HUMAN
Uniprot Description
MIOX: Belongs to the myo-inositol oxygenase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - inositol phosphate; EC 1.13.99.1; Oxidoreductase
Chromosomal Location of Human Ortholog: 22q13.3
Cellular Component: cytoplasm; inclusion body
Molecular Function: aldo-keto reductase activity; ferric iron binding; inositol oxygenase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen
Biological Process: inositol catabolic process
Similar Products
Product Notes
The MIOX miox (Catalog #AAA6320879) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIOX, NT (MIOX, ALDRL6, KSP32, RSOR, Inositol oxygenase, Aldehyde reductase-like 6, Kidney-specific protein 32, Myo-inositol oxygenase, Renal-specific oxidoreductase) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIOX can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MIOX miox for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIOX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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