Typical Testing Data/Standard Curve (for reference only)
Guinea Pig PTGES ELISA Kit | PTGES elisa kit
Guinea pig PTGES (Prostaglandin E Synthase, Microsomal)ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PTGES. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PTGES and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PTGES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PTGES. You can calculate the concentration of PTGES in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53 induced apoptosis. Knockout studies in mice suggest that this gene may contribute to the pathogenesis of collagen-induced arthritis and mediate acute pain during inflammatory responses. [provided by RefSeq, Jul 2008]
Uniprot Description
PTGES: Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Belongs to the MAPEG family.
Protein type: Membrane protein, multi-pass; Isomerase; Lipid Metabolism - arachidonic acid; Nuclear envelope; Membrane protein, integral; EC 5.3.99.3
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: endoplasmic reticulum membrane; membrane; nuclear envelope lumen; perinuclear region of cytoplasm; integral to membrane
Molecular Function: glutathione binding; prostaglandin-E synthase activity
Biological Process: negative regulation of cell proliferation; chronic inflammatory response; response to retinoic acid; response to cytokine stimulus; cyclooxygenase pathway; arachidonic acid metabolic process; response to lipopolysaccharide; signal transduction; prostaglandin biosynthetic process; response to calcium ion; response to organic cyclic substance; acute inflammatory response; prostaglandin metabolic process
Research Articles on PTGES
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Product Notes
The Guinea Pig PTGES ptges (Catalog #AAA2508741) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2508741 ELISA Kit recognizes Guinea Pig PTGES. It is sometimes possible for the material contained within the vial of "PTGES, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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