anti-Human, Mouse SUMO1 Antibody | anti-SUMO1 antibody

SUMO1 Antibody

Cat. Num. AAA8516323

• Gene Names: SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: SUMO1; SUMO1 Antibody; DAP-1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; SUMO-1; UBL1; anti-SUMO1 antibody

• Reactivity: Human, Mouse
• Specificity: SUMO1 Antibody detects endogenous levels of total SUMO1
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 101

• Applicable Applications for anti-SUMO1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide of human SUMO1
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts of A431cell line, using SUMO1 antibody.)

Related Product Information for anti-SUMO1 antibody

Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1,-2 and-3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1,-2 or-3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1,-2 or-3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1,-2 or-3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IkappaB-alpha to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease (8-11).

NCBI and Uniprot Product Information

• NCBI GI #: 54792065
• NCBI GeneID: 7341
• NCBI Accession #: NP_001005781.1
• NCBI GenBank Nucleotide #: NM_001005781.1
• UniProt Accession #: P63165; P55856; Q6FGG0; Q6NZ62; Q93068; A8MUS8; B2R4I5
• Molecular Weight: 12kDa
• NCBI Official Full Name: small ubiquitin-related modifier 1 isoform a
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 1
• NCBI Official Symbol: SUMO1
• NCBI Official Synonym Symbols: DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
• NCBI Protein Information: small ubiquitin-related modifier 1
• UniProt Protein Name: Small ubiquitin-related modifier 1
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO1
• UniProt Synonym Gene Names: SMT3C; SMT3H3; UBL1; SUMO-1; GMP1; Smt3C

NCBI Description

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed:24651376).