Human Equilibrative nucleoside transporter 1 ELISA Kit | SLC29A1 elisa kit

Human Equilibrative nucleoside transporter 1 ELISA Kit

Cat. Num. AAA288727

• Gene Names: SLC29A1; ENT1
• Reactivity: Human
• Synonyms: Equilibrative nucleoside transporter 1; Human Equilibrative nucleoside transporter 1 ELISA Kit; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative NBMPR-sensitive nucleoside transporter; Nucleoside; SLC29A1 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human ENT1. No significant cross-reactivity or interference was observed.
• Sequence Length: 456

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.065 ng/ml
• Intra-Assay CV: <=6.3%
• Inter-Assay CV: <=10.3%
• Recovery: 83%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC29A1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human equilibrative nucleoside transporter 1,ENT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to ENT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ENT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ENT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ENT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 118582262
• NCBI GeneID: 2030
• NCBI Accession #: NP_001071643.1
• NCBI GenBank Nucleotide #: NM_001078175.2
• UniProt Accession #: Q99808; Q5T9W9; Q9UJY2; B3KQV7; B3KQY5
• Molecular Weight: 58,963 Da
• NCBI Official Full Name: equilibrative nucleoside transporter 1 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 29 (equilibrative nucleoside transporter), member 1
• NCBI Official Symbol: SLC29A1
• NCBI Official Synonym Symbols: ENT1
• NCBI Protein Information: equilibrative nucleoside transporter 1
• UniProt Protein Name: Equilibrative nucleoside transporter 1
• Protein Family: Equilibrative nucleoside transporter
• UniProt Gene Name: SLC29A1
• UniProt Synonym Gene Names: ENT1; Equilibrative NBMPR-sensitive nucleoside transporter
• UniProt Entry Name: S29A1_HUMAN

NCBI Description

This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC29A1: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: apical plasma membrane; basolateral plasma membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: nucleoside transmembrane transporter activity

Biological Process: lactation; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; nucleoside transport; regulation of excitatory postsynaptic membrane potential; sleep; transmembrane transport; uridine transport