Human FOXO2 ELISA Kit | FOXO2 elisa kit

Human FOXO2 (Forkhead Box Protein O2) ELISA Kit

Cat. Num. AAA2501595

• Gene Names: FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• Reactivity: Human
• Synonyms: FOXO2; Human FOXO2 (Forkhead Box Protein O2) ELISA Kit; FOXO2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human FOXO2. No significant cross-reactivity or interference between Human FOXO2 and analogues was observed.
• Sequence Length: 673

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXO2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FOXO2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FOXO2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FOXO2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FOXO2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FOXO2. You can calculate the concentration of FOXO2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 42519916
• NCBI GeneID: 2309
• NCBI Accession #: NP_963853.1
• NCBI GenBank Nucleotide #: NM_201559.2
• UniProt Accession #: O43524; O15171; Q5T2I7; Q9BZ04; B4DVZ6; E1P5E6
• Molecular Weight: 48,406 Da
• NCBI Official Full Name: forkhead box protein O3
• NCBI Official Synonym Full Names: forkhead box O3
• NCBI Official Symbol: FOXO3
• NCBI Official Synonym Symbols: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• NCBI Protein Information: forkhead box protein O3; forkhead box O3A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• UniProt Protein Name: Forkhead box protein O3
• UniProt Gene Name: FOXO3
• UniProt Synonym Gene Names: FKHRL1; FOXO3A
• UniProt Entry Name: FOXO3_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.

Protein type: DNA-binding; Oncoprotein; Transcription factor; Autophagy

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin DNA binding; transcription factor activity; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; initiation of primordial ovarian follicle growth; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; antral ovarian follicle growth; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; ovulation from ovarian follicle; oocyte maturation; regulation of transcription from RNA polymerase II promoter; regulation of translation; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter