Typical Testing Data/Standard Curve (for reference only)
Rabbit FOXO3 ELISA Kit | FOXO3 elisa kit
Rabbit FOXO3 (Forkhead Box Protein O3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FOXO3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FOXO3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FOXO3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FOXO3. You can calculate the concentration of FOXO3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.
Protein type: DNA-binding; Oncoprotein; Transcription factor; Autophagy
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin DNA binding; transcription factor activity; protein kinase binding
Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; DNA damage response, signal transduction by p53 class mediator; nerve growth factor receptor signaling pathway; initiation of primordial ovarian follicle growth; positive regulation of erythrocyte differentiation; antral ovarian follicle growth; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; ovulation from ovarian follicle; glucose homeostasis; oocyte maturation; regulation of transcription from RNA polymerase II promoter; regulation of translation; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter
Research Articles on FOXO3
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Product Notes
The Rabbit FOXO3 foxo3 (Catalog #AAA2510961) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2510961 ELISA Kit recognizes Rabbit FOXO3. It is sometimes possible for the material contained within the vial of "FOXO3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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