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Immunohistochemistry (IHC) (Anti-MAPK4 / ERK4 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)

Rabbit anti-Human MAPK4 / ERK4 Polyclonal Antibody | anti-MAPK4 / ERK4 antibody

Anti-MAPK4 / ERK4 Antibody (aa1-326) IHC-plus

Gene Names
MAPK4; ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunoaffinity purified
Synonyms
MAPK4 / ERK4; Polyclonal Antibody; Anti-MAPK4 / ERK4 Antibody (aa1-326) IHC-plus; Rabbit Polyclonal (IgG) to Human MAPK4 / ERK4; Human MAPK4 / ERK4; ERK-4; Erk3-related; Erk4; MAP kinase isoform p63; PRKM4; RMNK2; MAP kinase 4; ERK3; MAPK 4; p63-MAPK; p63MAPK; anti-MAPK4 / ERK4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human MAPK4 / ERK4
Purity/Purification
Immunoaffinity purified
Form/Format
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Concentration
1 mg/ml (varies by lot)
Sequence Length
587
Applicable Applications for anti-MAPK4 / ERK4 antibody
Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC (1:100 - 1:1000)
IHC-P (7.5 ug/ml)
WB (1:500 - 1:3000)
Immunogen
MAPK4 / ERK4 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 326 of MAPK4 (SwissProt P31152). Percent identity by BLAST analysis: Human (100%); Mouse (95%).
Immunogen Description
Recombinant fragment corresponding to a region within amino acids 1 and 326 of MAPK4 (SwissProt P31152). Percent identity by BLAST analysis: Human (100%); Mouse (95%).
Immunogen Type
Recombinant protein
Antigen Modification
aa1-326
Target Species
Human
Preparation and Storage
Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-MAPK4 / ERK4 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)

Immunohistochemistry (IHC) (Anti-MAPK4 / ERK4 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)

Immunocytochemistry (ICC)

(Immunofluorescence of paraformaldehyde-fixed HeLa using MAPK4 antibody at 1:200 dilution.)

Immunocytochemistry (ICC) (Immunofluorescence of paraformaldehyde-fixed HeLa using MAPK4 antibody at 1:200 dilution.)

Western Blot (WB)

(Sample (30 ug whole cell lysate). A: H1299. 7.5% SDS PAGE. MAPK4 / ERK4 antibody diluted at 1:1000)

Western Blot (WB) (Sample (30 ug whole cell lysate). A: H1299. 7.5% SDS PAGE. MAPK4 / ERK4 antibody diluted at 1:1000)
Related Product Information for anti-MAPK4 / ERK4 antibody
MAPK4 Antibody, ERK-4 Antibody, Erk3-related Antibody, Erk4 Antibody, MAP kinase isoform p63 Antibody, PRKM4 Antibody, RMNK2 Antibody, MAP kinase 4 Antibody, ERK3 Antibody, MAPK 4 Antibody, p63-MAPK Antibody, p63MAPK Antibody Description: Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65,922 Da
NCBI Official Full Name
mitogen-activated protein kinase 4 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 4
NCBI Official Symbol
MAPK4
NCBI Official Synonym Symbols
ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
NCBI Protein Information
mitogen-activated protein kinase 4
UniProt Protein Name
Mitogen-activated protein kinase 4
UniProt Gene Name
MAPK4
UniProt Synonym Gene Names
ERK4; PRKM4; MAP kinase 4; MAPK 4; ERK-4; p63-MAPK
UniProt Entry Name
MK04_HUMAN

NCBI Description

Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

ERK4: a protein kinase of the MAPK family. Phosphorylation and activations leads to translocation into the nucleus where it phosphorylates nuclear targets.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: ATP binding; MAP kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: cell cycle; MAPKKK cascade; protein amino acid phosphorylation

Research Articles on MAPK4 / ERK4

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Product Notes

The MAPK4 / ERK4 mapk4 (Catalog #AAA249804) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MAPK4 / ERK4 Antibody (aa1-326) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK4 / ERK4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB). ICC (1:100 - 1:1000) IHC-P (7.5 ug/ml) WB (1:500 - 1:3000). Researchers should empirically determine the suitability of the MAPK4 / ERK4 mapk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK4 / ERK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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