Western Blot (WB)
(Western blot analysis of p63 MAPK using K562 whole cell lysates)
Rabbit anti-Human, Mouse p63 MAPK Polyclonal Antibody | anti-MAPK4 antibody
p63 MAPK Antibody
Gene Names
MAPK4; ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
p63 MAPK; Polyclonal Antibody; p63 MAPK Antibody; ERK-4; ERK3; Erk3 related; ERK4; Extracellular signal regulated kinase 4; Extracellular signal-regulated kinase 4; MAP kinase 4; MAP kinase isoform p63; MAPK 4; MAPK4; Mitogen activated protein kinase 4; Mitogen-activated protein kinase 4; MK04_HUMAN; p63-MAPK; p63MAPK; PRKM4; Protein kinase; mitogen-activated; 4; Protein-tyrosine kinase ERK3-related; RMNK2; anti-MAPK4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
p63 MAPK antibody detects endogenous levels of total p63 MAPK
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
376
Applicable Applications for anti-MAPK4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human p63 MAPK
Subcellular Location
Cytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5.
Tissue Specificity
High expression in heart and brain.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of p63 MAPK using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of p63 MAPK using K562 whole cell lysates)
Related Product Information for anti-MAPK4 antibody
Function: Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity).
Subunit Structure: Homodimer. Heterodimer with ERK3/MAPK6. Interacts with (via FRIEDE motif) MAPKAPK5 (By similarity).
Post-translational Modifications: Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
Similarity: The FRIEDE motif is required for docking MAPKAPK5.In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Subunit Structure: Homodimer. Heterodimer with ERK3/MAPK6. Interacts with (via FRIEDE motif) MAPKAPK5 (By similarity).
Post-translational Modifications: Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
Similarity: The FRIEDE motif is required for docking MAPKAPK5.In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 66 kDa
Predicted: 66 kDa
Predicted: 66 kDa
NCBI Official Full Name
mitogen-activated protein kinase 4 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase 4
NCBI Official Symbol
MAPK4
NCBI Official Synonym Symbols
ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
NCBI Protein Information
mitogen-activated protein kinase 4
UniProt Protein Name
Mitogen-activated protein kinase 4
UniProt Gene Name
MAPK4
UniProt Synonym Gene Names
ERK4; PRKM4; MAP kinase 4; MAPK 4; ERK-4; p63-MAPK
NCBI Description
Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle ().
Research Articles on MAPK4
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Product Notes
The MAPK4 mapk4 (Catalog #AAA9609113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p63 MAPK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p63 MAPK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the MAPK4 mapk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p63 MAPK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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